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Blood, 1 September 2006, Vol. 108, No. 5, pp. 1533-1541. Prepublished online as a Blood First Edition Paper on May 4, 2006; DOI 10.1182/blood-2005-12-012104. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2005-12-012104v1 108/5/1533    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bockamp, E. Articles by Eshkind, L. Search for Related Content PubMed PubMed Citation Articles by Bockamp, E. Articles by Eshkind, L. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 12, 2005 Accepted April 21, 2006 Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes and c-kit expressing lineage negative hematopoietic cells Ernesto Bockamp*, Cecilia Antunes, Marko Maringer, Rosario Heck, Katrin Presser, Sven Beilke, Svetlana Ohngemach, Rudiger Alt, Michael Cross, Rolf Sprengel, Udo Hartwig, Bernd Kaina, Steffen Schmitt, and Leonid Eshkind Institute of Toxicology/Mouse Genetics, Johannes Gutenberg-Universitat Mainz, Germany Department of Hematology/Oncology, University of Leipzig, Leipzig, Germany Max-Planck-Institute for Medical Research, Heidelberg, Germany Department of Hematology/Oncology, University Medical School, Johannes Gutenberg-Universitat Mainz FACS and Array Core Facility, Johannes Gutenberg-Universitat Mainz, Germany * Corresponding author; email: bockamp{at}mail.uni-mainz.de. The stem cell leukaemia gene SCL, also known as TAL-1, encodes a basic helix-loop-helix transcription factor expressed in erythroid, myeloid, megakaryocytic and hematopoietic stem cells. To be able to make use of the unique tissue-restricted and spatio-temporal expression pattern of the SCL gene, we have generated a knock-in mouse line containing the tTA-2S tetracycline transactivator under the control of SCL regulatory elements. Analysis of this mouse using different tetracycline-dependant reporter strains demonstrated that switchable transgene expression was restricted to erythrocytes, megakaryocytes, granulocytes and importantly to the c-kit-expressing and lineage negative cell fraction of the bone marrow. In addition, conditional transgene activation was also detected in a very minor population of endothelial cells and in the kidney. However, no activation of the reporter transgene was found in the brain of adult mice. These findings suggested that the expression of tetracycline-responsive reporter genes recapitulated the known endogenous expression pattern of SCL. Our data therefore demonstrate that exogenously inducible and reversible expression of selected transgenes in myeloid, megakaryocytic, erythroid, and c-kit-expressing lineage negative bone marrow cells can be directed through SCL regulatory elements. The SCL knock-in mouse presented here represents a powerful tool for studying normal and malignant hematopoiesis in vivo. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Hameyer, A. Loonstra, L. Eshkind, S. Schmitt, C. Antunes, A. Groen, E. Bindels, J. Jonkers, P. Krimpenfort, R. Meuwissen, et al. Toxicity of ligand-dependent Cre recombinases and generation of a conditional Cre deleter mouse allowing mosaic recombination in peripheral tissues Physiol Genomics, September 11, 2007; 31(1): 32 - 41. [Abstract] [Full Text] [PDF]

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