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Blood, 1 January 2007, Vol. 109, No. 1, pp. 168-175. Prepublished online as a Blood First Edition Paper on September 14, 2006; DOI 10.1182/blood-2005-12-020164. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2005-12-020164v1 109/1/168    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Suzuki, J.-i. Articles by Saito, T. Search for Related Content PubMed PubMed Citation Articles by Suzuki, J.-i. Articles by Saito, T. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 13, 2005 Accepted August 15, 2006 Actin cloud induced by LFA-1-mediated outside-in signals lowers the threshold for T cell activation Jun-ichiro Suzuki, Sho Yamasaki, Jennifer Wu, Gary A. Koretzky, and Takashi Saito* Department of Molecular Genetics, Graduate School of Medicine, Chiba University, Japan RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa, Japan University of Pennsylvania School of Medicine, Abramson Family Cancer Research Institute, PA, USA * Corresponding author; email: saito{at}rcai.riken.jp. The dynamic rearrangement of the actin cytoskeleton plays critical roles in TCR signaling and immunological synapse (IS) formation in T cells. Following actin rearrangement in T cells upon TCR stimulation, we found a unique ring-shaped reorganization of actin called "actin cloud," which was specifically induced by outside-in signals through LFA-1 engagement. Under T cell-APC interaction, the actin cloud is generated in the absence of antigen and localized at the center of the T cell-APC interface, where it accumulates LFA-1 and tyrosine-phosphorylated proteins. The LFA-1-induced actin cloud formation involves ADAP phosphorylation, LFA-1/ADAP assembly and JNK activation, and occurs independent of TCR and its proximal signaling. The formation of the actin cloud lowers the threshold for subsequent T cell activation. Thus, the actin cloud induced by LFA-1 engagement may serve as a possible platform for LFA-1-mediated co-stimulatory function for T cell activation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. G. Baker, C. J. Hsu, D. Lee, M. S. Jordan, J. S. Maltzman, D. A. Hammer, T. Baumgart, and G. A. Koretzky The Adapter Protein SLP-76 Mediates "Outside-In" Integrin Signaling and Function in T Cells Mol. Cell. 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