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Blood, 15 January 2007, Vol. 109, No. 2, pp. 661-669. Prepublished online as a Blood First Edition Paper on September 26, 2006; DOI 10.1182/blood-2005-12-023044. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2005-12-023044v1 109/2/661    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Varga, G. Articles by Grabbe, S. Search for Related Content PubMed PubMed Citation Articles by Varga, G. Articles by Grabbe, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 5, 2005 Accepted August 1, 2006 Active MAC-1 (CD11b/CD18) on DC is inhibitory for full T cell activation Georg Varga, Sandra Balkow, Martin K Wild, Andrea Stadtbaeumer, Mathias Krummen, Tobias Rothoeft, Tetsuya Higuchi, Stefan Beissert, Klaus Wethmar, Karin Scharffetter-Kochanek, Dietmar Vestweber, and Stephan Grabbe* Department of Dermatology, University of Muenster, Germany Center for Molecular Biology of Inflammation, University of Muenster, Germany Max-Delbrueck-Center for Molecular Medicine, Berlin-Buch, Germany Department of Dermatology, University of Ulm, Germany Max-Planck-Institute for Molecular Biomedicine, Muenster, Germany Department of Dermatology, University of Essen, Germany * Corresponding author; email: grabbe{at}med.uni-essen.de. 2 integrins are important for transendothelial migration of leukocytes as well as for T cell activation during antigen presentation. Despite abundant expression of 2 integrins on APC, their functional relevance for antigen presentation is completely unclear. We show here that DC from CD18-deficient mice, which lack all functional 2 integrins, have no defect in antigen presentation. Moreover, DC from normal mice express inactive 2 integrins that do not become activated upon contact with T cells, at least in vitro. Pharmacological activation of 2 integrins on DC results in a significant reduction of their T cell activating capacity. This effect is mediated by Mac-1 (CD11b/CD18) on DC since it could be reversed via blocking antibodies against CD18 and CD11b. Furthermore, the antigen presenting capacity of macrophages, that express constitutively active 2 integrins, is significantly enhanced upon Mac-1 blockade. We therefore conclude that active CD11b/CD18 (Mac-1) on APC directly inhibits T cell activation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? 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