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Blood, 15 September 2006, Vol. 108, No. 6, pp. 1865-1867. Prepublished online as a Blood First Edition Paper on May 25, 2006; DOI 10.1182/blood-2006-01-013540. This Article Full Text (PDF) All Versions of this Article: blood-2006-01-013540v1 108/6/1865    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lippert, E. Articles by Hermouet, S. Search for Related Content PubMed PubMed Citation Articles by Lippert, E. Articles by Hermouet, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 24, 2006 Accepted May 8, 2006 The JAK2-V617F mutation is frequently present at diagnosis in patients with essential thrombocythemia and polycythemia vera Eric Lippert, Marjorie Boissinot, Robert Kralovics, Francois Girodon, Irene Dobo, Vincent Praloran, Nathalie Boiret-Dupre, Radek C Skoda, and Sylvie Hermouet* Laboratoire d'Hematologie du CHU de Bordeaux, Bordeaux, France INSERM U601, Nantes, France Department of Research, Experimental Hematology, University Hospital Basel, Switzerland Laboratoire d'Hematologie du CHU de Dijon, Dijon, France Laboratoire d'Hematologie du CHU d'Angers, Angers, France Laboratoire d'Hematologie du CHU de Clermont-Ferrand, Clermont-Ferrand, France INSERM U601, Nantes, France; Laboratoire d'Hematologie du CHU de Nantes, France * Corresponding author; email: sylvie.hermouet{at}chu-nantes.fr. We determined the allelic frequency of the JAK2-V617F mutation in DNA and assessed the expression levels of the mutant and wild type JAK2 mRNA in granulocytes from 60 patients with essential thrombocythemia (ET) and 62 patients with polycythemia vera (PV) at the time of diagnosis. Using allele-specific, quantitative PCR, we detected JAK2-V617F in 75% of ET and 97% of PV at diagnosis. The total JAK2 mRNA levels were elevated in ET, PV and secondary and idiopathic erythrocytosis, suggesting that hyperactive hematopoiesis alters JAK2 expression. The expression levels of JAK2-V617F mRNA were variable but strongly correlated with the allelic ratio of JAK2-V617F determined in DNA. Thus, differences in JAK2-V617F expression, markedly lower in ET than in PV, reflected different percentages of granulocytes carrying the mutation. Moreover, allelic ratios > 50% JAK2-V617F, indicating the presence of granulocytes homozygous for JAK2-V617F, were found in 70% of PV at diagnosis but never in ET. 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