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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2399-2405.
Prepublished online as a Blood First Edition Paper on November 7, 2006; DOI 10.1182/blood-2006-01-030643.
 Previous Article | Next Article 
Submitted January 17, 2006
Accepted October 25, 2006
CD34 cells from trisomy 8 myelodysplastic syndrome (MDS)
patients express early apoptotic markers but avoid
programmed cell death by upregulation of anti-apoptotic
proteins
Elaine M Sloand*, Loretta Pfannes, Gubin Chen, Simant Shah, Elena Solomou, John Barrett, and Neal S Young
National Heart Lung and Blood Institute, NIH, United States
* Corresponding author; email: sloande{at}nhlbi.nih.gov.
CD34 cells from patients with trisomy 8 MDS are distinguished from other MDS and from normal hematopoietic cells by their pronounced expression of apoptotic markers. Paradoxically, trisomy 8 clones can persist in patients with bone marrow failure and expand following immunosuppression. We previously demonstrated upregulation of c-myc and CD1 by microarray analysis. Here we confirmed these findings by real-time PCR, demonstrated upregulation of survivin, c-myc and CD1 protein expression, and documented comparable colony formation by annexin+ trisomy 8- CD34+ and annexin- CD34 cells. There were low levels of DNA degradation in annexin+ trisomy 8 CD34 cells which were comparable to annexin- CD34 cells. Trisomy 8 cells were resistant to apoptosis induced by gamma irradiation. Knock-down of survivin by siRNA resulted in preferential loss of trisomy 8 cells. These results suggest that trisomy 8 cells undergo incomplete apoptosis and are capable nonetheless of colony formation and growth.
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