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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2389-2398.
Prepublished online as a Blood First Edition Paper on November 7, 2006; DOI 10.1182/blood-2006-02-003087.
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Submitted February 9, 2006
Accepted October 25, 2006
The transcription factors, Scl and Lmo2, act together during development of the haemangioblast in zebrafish
Lucy J Patterson, Martin Gering, Craig E Eckfeldt, Anthony R Green, Catherine M Verfaillie, Stephen C Ekker, and Roger Patient*
Oxford University, John Radcliffe Hospital, United Kingdom
University of Nottingham, Queen's Medical Centre, United Kingdom
University of Minnesota, United States
University of Cambridge, United Kingdom
* Corresponding author; email: roger.patient{at}imm.ox.ac.uk.
The transcription factors, Scl and Lmo2, are crucial for development of all blood. An important early requirement for Scl in endothelial development has also been revealed recently in zebrafish embryos, supporting previous findings in scl-/- embryoid bodies. Scl depletion culminates most notably in failure of dorsal aorta formation, potentially revealing a role in the formation of haemogenic endothelium. We now present evidence that the requirements for Lmo2 in zebrafish embryos are essentially the same as for Scl. The expression of important haematopoietic regulators is lost, reduced or delayed, pan-endothelial gene expression is downregulated and aorta-specific marker expression is lost. The close similarity of the phenotypes for Scl and Lmo2 suggest that they perform these early functions in haemangioblast development within a multi-protein complex, as shown for erythropoiesis. Consistent with this, we find that Scl morphants cannot be rescued by a non-Lmo2 binding form of Scl but can be rescued by non-DNA binding forms, suggesting tethering to target genes through DNA-binding partners linked via Lmo2. Interestingly, unlike other haematopoietic regulators, the Scl/Lmo2 complex does not appear to autoregulate as neither gene's expression is affected by depletion of the other. Thus, expression of these critical regulators is dependent on continued expression of upstream regulators, which may include cell extrinsic signals.
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