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Blood, 15 August 2006, Vol. 108, No. 4, pp. 1413-1420. Prepublished online as a Blood First Edition Paper on April 25, 2006; DOI 10.1182/blood-2006-02-004341. This Article Full Text (PDF) All Versions of this Article: blood-2006-02-004341v1 108/4/1413    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nakao, A. Articles by Murase, N. Search for Related Content PubMed PubMed Citation Articles by Nakao, A. Articles by Murase, N. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 16, 2006 Accepted April 11, 2006 Simultaneous bone marrow and intestinal transplantation promotes marrow-derived hematopoietic stem cell engraftment and chimerism Atsunori Nakao, Hideyoshi Toyokawa, Kei Kimizuka, Michael A. Nalesnik, Isao Nozaki, Robert J Bailey, Anthony J Demetris, Thomas E Starzl, and Noriko Murase* Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Dept. of Surgery Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Dept. of Pathology Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Dept. of Surgery * Corresponding author; email: murase{at}pitt.edu. Organ allografts have been shown to provide a syngenic microenvironment for organ-based donor hematopoietic stem cells to maintain long-lasting chimerism after transplantation. We hypothesized that organ allografts would also support engraftment and hematopoiesis of adjunctively infused donor marrow stem cells, syngenic to organ grafts, in non-myeloablated recipients. In BN-to-LEW and GFP-to-ACI rat combinations, donor bone marrow (BM) infusion together with small intestinal transplantation (SITx) under short course tacrolimus immunosuppression resulted in persistent macrochimerism (>5%) for 150d. In contrast, after BM infusion or SITx alone, chimerism was temporary and disappeared by 100d. Y-chromosome PCR in sex-mismatched male BM plus female intestine or female BM plus male intestine transplantation into female recipients suggested that persistent macrochimerism was derived from infused BM. BM infusion together with lymphoid-depleted intestinal grafts also supported macrochimerism development; however third-party intestinal grafts did not. After GFP+BM plus WT SITx into ACI, large numbers of GFP+ leukocytes were found in WT intestinal grafts. Isolated cells from WT intestinal grafts developed GFP+ CFU-C and propagated multilineage GFP+ leukocytes when adoptively transferred into lethally irradiated WT recipients. These findings suggest that intestinal allograft supports simultaneously infused donor (syngenic to organ grafts) marrow stem cell engraftment, differentiation and persistence of chimerism. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Tomiyama, N. Murase, D. B. Stolz, H. Toyokawa, D. R. O'Donnell, D. M. Smith, J. R. Dudas, J. P. Rubin, and K. G. Marra Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells into Adipose Tissue Stem Cells, February 1, 2008; 26(2): 330 - 338. [Abstract] [Full Text] [PDF]

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