SEARCH:   Advanced

Blood, 1 November 2006, Vol. 108, No. 9, pp. 3152-3160. Prepublished online as a Blood First Edition Paper on July 13, 2006; DOI 10.1182/blood-2006-02-005322. This Article Full Text (PDF) Supplemental Table All Versions of this Article: blood-2006-02-005322v1 108/9/3152    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dicker, F. Articles by Schoch, C. Search for Related Content PubMed PubMed Citation Articles by Dicker, F. Articles by Schoch, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 22, 2006 Accepted June 26, 2006 Immunostimulatory oligonucleotide-induced metaphase cytogenetics detects chromosomal aberrations in 80% of CLL patients: A study of 132 CLL cases with correlation to FISH, IgVH status and CD38 expression Frank Dicker*, Susanne Schnittger, Torsten Haferlach, Wolfgang Kern, and Claudia Schoch MLL Munich Leukemia Diagnostics GmbH, Munich, Germany * Corresponding author; email: frank.dicker{at}mll-online.com. Compared to fluorescence in situ hybridization (FISH) conventional metaphase cytogenetics plays only a minor prognostic role in chronic lymphocytic leukemia (CLL) so far due to technical problems resulting from limited proliferation of CLL cells in vitro. Here we present a simple method for in vitro stimulation of CLL cells which overcomes this limitation. In our unselected patient population 125/132 cases could be successfully stimulated for metaphase generation by culture with the immunostimulatory CpG-oligonucleotide DSP30 plus interleukin 2. 101/125 cases showed chromosomal aberrations. The aberration rate is comparable to the rate detected by parallel interphase FISH. Conventional cytogenetics detected additional aberrations in 47 patients compared to FISH analysis. A complex aberrant karyotype, defined as 3 aberrations, was detected in 30/125 patients compared to only one such case as defined by FISH. Conventional cytogenetics frequently detected balanced and unbalanced translocations. A significant correlation of the poor prognosis unmutated IgVH status with unbalanced translocations and of the likewise poor prognosis CD38 expression to balanced translocations and complex aberrant karyotype was found. We demonstrate that FISH analysis underestimates the complexity of chromosomal aberrations in CLL. Therefore, conventional cytogenetics may define subgroups of patients with high risk of progression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: V. Grubor, A. Krasnitz, J. E. Troge, J. L. Meth, B. Lakshmi, J. T. Kendall, B. Yamrom, G. Alex, D. Pai, N. Navin, et al. Novel genomic alterations and clonal evolution in chronic lymphocytic leukemia revealed by representational oligonucleotide microarray analysis (ROMA) Blood, February 5, 2009; 113(6): 1294 - 1303. [Abstract] [Full Text] [PDF] T. J. Kipps Genomic complexity in chronic lymphocytic leukemia Blood, September 1, 2008; 112(5): 1550 - 1550. [Full Text] [PDF] L. Kujawski, P. Ouillette, H. Erba, C. Saddler, A. Jakubowiak, M. Kaminski, K. Shedden, and S. N. Malek Genomic complexity identifies patients with aggressive chronic lymphocytic leukemia Blood, September 1, 2008; 112(5): 1993 - 2003. [Abstract] [Full Text] [PDF] F. Dicker, C. Haferlach, W. Kern, T. Haferlach, and S. Schnittger Trisomy 13 is strongly associated with AML1/RUNX1 mutations and increased FLT3 expression in acute myeloid leukemia Blood, August 15, 2007; 110(4): 1308 - 1316. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020