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Blood, 1 January 2007, Vol. 109, No. 1, pp. 343-352. Prepublished online as a Blood First Edition Paper on August 29, 2006; DOI 10.1182/blood-2006-03-006569. This Article Full Text (PDF) Erratum (v109,p4616) All Versions of this Article: blood-2006-03-006569v1 109/1/343    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fraser, S. T. Articles by Baron, M. H. Search for Related Content PubMed PubMed Citation Articles by Fraser, S. T. Articles by Baron, M. H. Related Collections Related Articles in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 1, 2006 Accepted August 9, 2006 Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell surface antigen expression Stuart T. Fraser, Joan Isern, and Margaret H. Baron* Department of Medicine, Mount Sinai School of Medicine, New York, NY, USA * Corresponding author; email: margaret.baron{at}mssm.edu. Primitive erythroblasts (EryP) are the first hematopoietic cell type to form during mammalian embryogenesis and emerge within the blood islands of the yolk sac. Large, nucleated EryP begin to circulate around midgestation, when connections between yolk sac and embryonic vasculature mature. Two to three days later, small cells of the definitive erythroid lineage (EryD) begin to differentiate within the fetal liver and rapidly outnumber EryP in the circulation. The development and maturation of EryP remain poorly defined. Our analysis of embryonic blood at different stages reveals a stepwise developmental progression within the EryP lineage from E9.5 to E12.5. Thereafter, EryD are also present in the bloodstream and the two lineages are not easily distinguished. We have generated a transgenic mouse line in which the human -globin gene promoter drives expression of green fluorescent protein exclusively within the EryP lineage. Here we have used this line to characterize changes in cell morphology and surface marker expression as EryP mature and to track EryP numbers and enucleation throughout gestation. This study identifies previously unrecognized synchronous developmental stages leading to the maturation of EryP in the mouse embryo. Unexpectedly, we find that EryP are a stable cell population that persists through the end of gestation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Articles in Blood Online: AKT induces erythroid-cell maturation of JAK2-deficient fetal liver progenitor cells and is required for Epo regulation of erythroid-cell differentiation Saghi Ghaffari, Claire Kitidis, Wei Zhao, Dragan Marinkovic, Mark D. Fleming, Biao Luo, Joseph Marszalek, and Harvey F. Lodish Blood 2006 107: 1888-1891. [Abstract] [Full Text] [PDF] Yolk sac–derived primitive erythroblasts enucleate during mammalian embryogenesis Paul D. Kingsley, Jeffrey Malik, Katherine A. Fantauzzo, and James Palis Blood 2004 104: 19-25. [Abstract] [Full Text] [PDF] This article has been cited by other articles: H. Beauchemin and M. Trudel Evidence for a Bigenic Chromatin Subdomain in Regulation of the Fetal-to-Adult Hemoglobin Switch Mol. Cell. Biol., March 15, 2009; 29(6): 1635 - 1648. [Abstract] [Full Text] [PDF] S.-J. Lu, Q. Feng, J. S. Park, L. Vida, B.-S. Lee, M. Strausbauch, P. J. Wettstein, G. R. Honig, and R. Lanza Biologic properties and enucleation of red blood cells from human embryonic stem cells Blood, December 1, 2008; 112(12): 4475 - 4484. [Abstract] [Full Text] [PDF] N. C. Dubois, C. Adolphe, A. Ehninger, R. A. Wang, E. J. Robertson, and A. Trumpp Placental rescue reveals a sole requirement for c-Myc in embryonic erythroblast survival and hematopoietic stem cell function Development, July 15, 2008; 135(14): 2455 - 2465. [Abstract] [Full Text] [PDF] J. Isern, S. T. Fraser, Z. He, and M. H. Baron The fetal liver is a niche for maturation of primitive erythroid cells PNAS, May 6, 2008; 105(18): 6662 - 6667. [Abstract] [Full Text] [PDF] K. E. McGrath, P. D. Kingsley, A. D. Koniski, R. L. Porter, T. P. Bushnell, and J. Palis Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus Blood, February 15, 2008; 111(4): 2409 - 2417. [Abstract] [Full Text] [PDF] P. Basu, T. K. Lung, W. Lemsaddek, T. G. Sargent, D. C. Williams Jr, M. Basu, L. C. Redmond, J. B Lingrel, J. L. Haar, and J. A. Lloyd EKLF and KLF2 have compensatory roles in embryonic {beta}-globin gene expression and primitive erythropoiesis Blood, November 1, 2007; 110(9): 3417 - 3425. [Abstract] [Full Text] [PDF]

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