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Blood, 1 January 2007, Vol. 109, No. 1, pp. 219-227. Prepublished online as a Blood First Edition Paper on August 29, 2006; DOI 10.1182/blood-2006-03-007153. This Article Full Text (PDF) All Versions of this Article: blood-2006-03-007153v1 109/1/219    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stowell, S. Articles by Cummings, R. D Search for Related Content PubMed PubMed Citation Articles by Stowell, S. Articles by Cummings, R. D Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 7, 2006 Accepted August 9, 2006 Human Galectins-1, -2, and -4 induce surface exposure of phosphatidylserine in activated human neutrophils but not activated T cells Sean Stowell, Sougata Karmakar, Caleb Stowell, Marcelo Dias-Baruffi, Rodger P McEver, and Richard D Cummings* Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK,USA * Corresponding author; email: rdcummi{at}emory.edu. Cellular turnover is associated with exposure of surface phosphatidylserine (PS) in apoptotic cells, leading to their phagocytic recognition and removal. But recent studies indicate that surface PS exposure is not always associated with apoptosis. Here we show that several members of the human galectin family of glycan binding proteins (galectins-1, -2, and -4) induce PS exposure in a carbohydrate-dependent fashion in activated, but not resting, human neutrophils and in several leukocyte cell lines. PS exposure is not associated with apoptosis in activated neutrophils. The exposure of PS in cell lines treated with these galectins is sustained and does not affect cell viability. Unexpectedly, these galectins bind well to activated T-lymphocytes, but do not induce either PS exposure or apoptosis, indicating that galectin effects are cell specific. These results suggest novel immunoregulatory contribution of galectins in regulating leukocyte turnover independently of apoptosis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Saravanan, Z. Cao, S. R Head, and N. 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[Abstract] [Full Text] [PDF] S. R. Stowell, S. Karmakar, C. M. Arthur, T. Ju, L. C. Rodrigues, T. B. Riul, M. Dias-Baruffi, J. Miner, R. P. McEver, and R. D. Cummings Galectin-1 Induces Reversible Phosphatidylserine Exposure at the Plasma Membrane Mol. Biol. Cell, March 1, 2009; 20(5): 1408 - 1418. [Abstract] [Full Text] [PDF] S. R. Stowell, M. Cho, C. L. Feasley, C. M. Arthur, X. Song, J. K. Colucci, S. Karmakar, P. Mehta, M. Dias-Baruffi, R. P. McEver, et al. Ligand Reduces Galectin-1 Sensitivity to Oxidative Inactivation by Enhancing Dimer Formation J. Biol. Chem., February 20, 2009; 284(8): 4989 - 4999. [Abstract] [Full Text] [PDF] N. Nishi, A. Abe, J. Iwaki, H. Yoshida, A. Itoh, H. Shoji, S. Kamitori, J. Hirabayashi, and T. Nakamura Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1 Glycobiology, December 1, 2008; 18(12): 1065 - 1073. [Abstract] [Full Text] [PDF] D. G. Cerri, L. C. Rodrigues, S. R. Stowell, D. D. Araujo, M. C. Coelho, S. R. Oliveira, J. C. S. Bizario, R. D. Cummings, M. Dias-Baruffi, and M. C. R. Costa Degeneration of dystrophic or injured skeletal muscles induces high expression of Galectin-1 Glycobiology, November 1, 2008; 18(11): 842 - 850. [Abstract] [Full Text] [PDF] C.-M. Tsai, Y.-K. Chiu, T.-L. Hsu, I-Y. Lin, S.-L. Hsieh, and K.-I Lin Galectin-1 Promotes Immunoglobulin Production during Plasma Cell Differentiation J. Immunol., October 1, 2008; 181(7): 4570 - 4579. [Abstract] [Full Text] [PDF] S. Karmakar, S. R Stowell, R. D Cummings, and R. P McEver Galectin-1 signaling in leukocytes requires expression of complex-type N-glycans Glycobiology, October 1, 2008; 18(10): 770 - 778. [Abstract] [Full Text] [PDF] S. R. Stowell, C. M. Arthur, K. A. Slanina, J. R. Horton, D. F. Smith, and R. D. Cummings Dimeric Galectin-8 Induces Phosphatidylserine Exposure in Leukocytes through Polylactosamine Recognition by the C-terminal Domain J. Biol. Chem., July 18, 2008; 283(29): 20547 - 20559. 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