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Blood, 15 November 2006, Vol. 108, No. 10, pp. 3387-3396. Prepublished online as a Blood First Edition Paper on July 13, 2006; DOI 10.1182/blood-2006-03-009266. This Article Full Text (PDF) All Versions of this Article: blood-2006-03-009266v1 108/10/3387    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bozza, S. Articles by Romani, L. Search for Related Content PubMed PubMed Citation Articles by Bozza, S. Articles by Romani, L. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 10, 2006 Accepted July 3, 2006 Pentraxin 3 protects from MCMV infection and reactivation through TLR sensing pathways leading to IRF3 activation Silvia Bozza, Francesco Bistoni, Roberta Gaziano, Lucia Pitzurra, Teresa Zelante, Pierluigi Bonifazi, Katia Perruccio, Silvia Bellocchio, Mariella Neri, Anna Maria Iorio, Giovanni Salvatori, Rita De Santis, Mario Calvitti, Andrea Doni, Cecilia Garlanda, Alberto Mantovani, and Luigina Romani* Microbiology Section, University of Perugia Sigma-Tau, Pomezia, Roma, Istituto Clinico Humanitas, Milano Istituto Clinico Humanitas, Milano, * Corresponding author; email: lromani{at}unipg.it. Reactivation of latent human cytomegalovirus (HCMV) following allogeneic transplantation is a major cause of morbidity and mortality and predisposes to severe complications, including superinfection by Aspergillus spp. Antimicrobial polypeptides, including defensins and mannan-binding lectin, are known to block viral fusion by cross-linking sugars on cell surface. Pentraxin 3 (PTX3), a member of the long pentraxin family, successfully restored antifungal immunity in experimental hematopoietic transplantation. We assessed here whether PTX3 binds HCMV and murine virus (MCMV) and the impact on viral infectivity and superinfection in vivo. We found that PTX3 bound both viruses, reduced viral entry and infectivity in vitro and protected from MCMV primary infection and reactivation as well as Aspergillus superinfection. This occurred through the activation of Interferon regulatory factor 3 (IRF3) in dendritic cells via the TLR9/MyD88-independent viral recognition sensing and the promotion of the IL-12/IFN--dependent effector pathway. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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