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Blood, 15 October 2006, Vol. 108, No. 8, pp. 2850-2856. Prepublished online as a Blood First Edition Paper on June 27, 2006; DOI 10.1182/blood-2006-03-010207. This Article Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2006-03-010207v1 108/8/2850    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Morita, Y. Articles by Nakauchi, H. Search for Related Content PubMed PubMed Citation Articles by Morita, Y. Articles by Nakauchi, H. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 15, 2006 Accepted June 9, 2006 Non - side-population hematopoietic stem cells in mouse bone marrow Yohei Morita, Hideo Ema*, Satoshi Yamazaki, and Hiromitsu Nakauchi Laboratory of Stem Cell Therapy, Institute of Medical Science, University of Tokyo, Japan * Corresponding author; email: hema{at}ims.u-tokyo.ac.jp. Most hematopoietic stem cells (HSCs) are assumed to reside in the so-called side population (SP) in adult mouse bone marrow (BM). We report the coexistence of non-SP HSCs that do not significantly differ from SP HSCs in numbers, capacities, and cell cycle states. When stained with Hoechst 33342 dye, the CD34-/lowc-Kit+Sca-1+lineage marker- (CD34-KSL) cell population, highly enriched in mouse HSCs, was almost equally divided into the SP and the main population (MP) that represents non-SP cells. Competitive repopulation assays with single or 30 SP- or MP-CD34-KSL cells found similar degrees of repopulating activity and frequencies of repopulating cells for these populations. Secondary transplantation detected self-renewal capacity in both populations. SP analysis of BM cells from primary recipient mice suggested that the SP and MP phenotypes are interconvertible. Cell cycle analysis revealed that CD34-KSL cells were in a quiescent state and showed uniform cell cycle kinetics, regardless of whether they were in the SP or MP. Bcrp-1 expression was similarly detected in SP- and MP-CD34-KSL cells, suggesting that the SP phenotype is regulated not only by Bcrp-1, but also by other factors. The SP phenotype does not specify all HSCs; its identity with stem cell function thus is unlikely. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Jiang, Q. Qiu, A. Khanna, N. W. Todd, J. Deepak, L. Xing, H. Wang, Z. Liu, Y. Su, S. A. Stass, et al. Aldehyde Dehydrogenase 1 Is a Tumor Stem Cell-Associated Marker in Lung Cancer Mol. Cancer Res., March 1, 2009; 7(3): 330 - 338. [Abstract] [Full Text] [PDF] J. Mori, Y. Ishihara, K. Matsuo, H. Nakajima, N. Terada, K. Kosaka, Z. Kizaki, and T. Sugimoto Hematopoietic Contribution to Skeletal Muscle Regeneration in Acid {alpha}-Glucosidase Knockout Mice J. Histochem. Cytochem., September 1, 2008; 56(9): 811 - 817. [Abstract] [Full Text] [PDF]

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