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Blood, 15 September 2006, Vol. 108, No. 6, pp. 2006-2012. Prepublished online as a Blood First Edition Paper on May 25, 2006; DOI 10.1182/blood-2006-03-011536. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-03-011536v1 108/6/2006    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yang, X. Articles by Zhang, K. Search for Related Content PubMed PubMed Citation Articles by Yang, X. Articles by Zhang, K. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 21, 2006 Accepted May 8, 2006 Association of Ig/BCL6 translocations with germinal center B-lymphocytes in human lymphoid tissues: Implications for malignant transformation Xuwei Yang, Koutetsu Lee, Jonathan Said, Xun Gong, and Ke Zhang* Department of Medicine, David Geffen School of Medicine at UCLA Department of Pathology, David Geffen School of Medicine at UCLA * Corresponding author; email: kzhang{at}mednet.ucla.edu. Chromosomal translocations (CT) between immunoglobulin (Ig) genes and the BCL6 proto-oncogene are frequently associated with diffuse large B cell lymphomas (DLBCL) and follicular lymphomas (FL) and are implicated in the development of these lymphomas. However, whether Ig/BCL6 translocation per se is sufficient to drive malignant transformation is not clear. To understand the biology of Ig/BCL6 translocated cells prior to their malignant transformation, we developed a system capable of detecting 1-3 Igµ/BCL6 CT cells in one million mixed cells through the detection of chimeric Im-BCL6E2 and BCL6E1-Cm1 transcripts that reflect reciprocal Igµ/BCL6 translocations. The chimeric transcripts that existed in the vast majority of normal lymphoid tissues are due to Igµ/BCL6 CT and were not generated from trans-splicing. Both Iµ-BCL6E2 and BCL6E1-Cµ1 transcripts were co-expressed in the same cell populations. The Ig/BCL6 recombination junctions themselves were isolated from B cell subpopulations expressing the Iµ-BCL6 transcripts. The appearance of Igµ/BCL6 CT was associated with cells expressing germinal center but not naive B cell markers. This study shows that Ig/BCL6 translocations occur in germinal center stage B cells in normal humans, and that Ig/BCL6 CTs per se are not likely sufficient to cause the malignant transformation in the context of human B cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Jardin, P. Ruminy, J.-P. Kerckaert, F. Parmentier, J.-M. Picquenot, S. Quief, C. Villenet, G. Buchonnet, M. Tosi, T. Frebourg, et al. Detection of somatic quantitative genetic alterations by multiplex polymerase chain reaction for the prediction of outcome in diffuse large B-cell lymphomas Haematologica, April 1, 2008; 93(4): 543 - 550. [Abstract] [Full Text] [PDF]

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