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Blood, 1 April 2007, Vol. 109, No. 7, pp. 2718-2726. Prepublished online as a Blood First Edition Paper on November 30, 2006; DOI 10.1182/blood-2006-03-012500. This Article Full Text (PDF) All Versions of this Article: blood-2006-03-012500v1 109/7/2718    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Caselli, E. Articles by Santoro, M. G. Search for Related Content PubMed PubMed Citation Articles by Caselli, E. Articles by Santoro, M. G. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 23, 2006 Accepted November 11, 2006 Human herpesvirus 8 acute infection of endothelial cells induces monocyte chemoattractant protein 1-dependent capillary-like structure formation: role of the IKK/NF-B pathway Elisabetta Caselli, Simona Fiorentini, Carla Amici, Dario Di Luca, Arnaldo Caruso, and M. Gabriella Santoro* Department of Experimental and Diagnostic Medicine, University of Ferrara, Ferrara, Italy Department of Experimental and Applied Medicine, University of Brescia, Brescia, Italy Department of Biology, University of Rome Tor Vergata, Rome, Italy * Corresponding author; email: santoro{at}bio.uniroma2.it. Human herpesvirus 8 (HHV-8) is considered the causative agent of Kaposi's sarcoma, a highly vascularized neoplasm characterized by spindle-shape cells of endothelial origin and inflammatory cell infiltration. HHV-8 cell-transforming ability has been associated with the activation of NF-B, a nuclear factor playing a pivotal role in promoting inflammation and cell proliferation; however, little is known on NF-B activation during acute HHV-8 infection. In the present report, we utilized a recently established in vitro model of HHV-8 acute productive infection in endothelial cells to investigate the effect of HHV-8 on NF-B activity and function. HHV-8 was found to rapidly and potently induce NF-B activity in endothelial cells via stimulation of the IB kinase (IKK). Following IKK activation, HHV-8 selectively triggered the production of high levels of monocyte chemoattractant protein-1 (MCP-1), while it did not affect the expression of other NF-B-dependent proinflammatory proteins, including TNF, interleukin-8 and RANTES. Deletion of NF-B-binding sites in the MCP-1 enhancer resulted in significant inhibition of HHV-8-induced transcription. Furthermore, MCP-1 production was accompanied by virus-induced capillary-like structure formation at early stages of infection. The results suggest that HHV-8-induced MCP-1 may play an important role in promoting inflammation and pathogenic angiogenesis typical of HHV-8-associated lesions. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Totally tubular: virally induced endothelial tube formation Philip E. Pellett Blood 2007 109: 2669-2670. [Full Text] [PDF] This article has been cited by other articles: J. F. Rossignol, S. La Frazia, L. Chiappa, A. Ciucci, and M. G. Santoro Thiazolides, a New Class of Anti-influenza Molecules Targeting Viral Hemagglutinin at the Post-translational Level J. Biol. Chem., October 23, 2009; 284(43): 29798 - 29808. [Abstract] [Full Text] [PDF] V. Punj, H. Matta, S. Schamus, T. Yang, Y. Chang, and P. M. Chaudhary Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-{kappa}B activation Blood, May 28, 2009; 113(22): 5660 - 5668. [Abstract] [Full Text] [PDF] Y. Izumiya, C. Izumiya, D. Hsia, T. J. Ellison, P. A. Luciw, and H.-J. Kung NF-{kappa}B Serves as a Cellular Sensor of Kaposi's Sarcoma-Associated Herpesvirus Latency and Negatively Regulates K-Rta by Antagonizing the RBP-J{kappa} Coactivator J. Virol., May 1, 2009; 83(9): 4435 - 4446. [Abstract] [Full Text] [PDF] T. Aoki, H. Kataoka, R. Ishibashi, K. Nozaki, K. Egashira, and N. Hashimoto Impact of Monocyte Chemoattractant Protein-1 Deficiency on Cerebral Aneurysm Formation Stroke, March 1, 2009; 40(3): 942 - 951. [Abstract] [Full Text] [PDF] V. Fonsato, S. Buttiglieri, M. Chiara Deregibus, B. Bussolati, E. Caselli, D. Di Luca, and G. Camussi PAX2 expression by HHV-8-infected endothelial cells induced a proangiogenic and proinvasive phenotype Blood, March 1, 2008; 111(5): 2806 - 2815. [Abstract] [Full Text] [PDF] S. Efklidou, R. Bailey, N. Field, M. Noursadeghi, and M. K. Collins vFLIP from KSHV inhibits anoikis of primary endothelial cells J. Cell Sci., February 15, 2008; 121(4): 450 - 457. [Abstract] [Full Text] [PDF] K.-P. Tse, N.-M. Tsang, K.-D. Chen, H.-P. Li, Y. Liang, C. Hsueh, K.-P. Chang, J.-S. Yu, S.-P. Hao, L.-L. Hsieh, et al. MCP-1 Promoter Polymorphism at 2518 Is Associated with Metastasis of Nasopharyngeal Carcinoma after Treatment Clin. Cancer Res., November 1, 2007; 13(21): 6320 - 6326. [Abstract] [Full Text] [PDF]

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