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Blood, 15 December 2006, Vol. 108, No. 13, pp. 4018-4024. Prepublished online as a Blood First Edition Paper on August 22, 2006; DOI 10.1182/blood-2006-03-012872. This Article Full Text (PDF) Supplemental Table and Figures All Versions of this Article: blood-2006-03-012872v1 108/13/4018    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ema, M. Articles by Takahashi, S. Search for Related Content PubMed PubMed Citation Articles by Ema, M. Articles by Takahashi, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 27, 2006 Accepted August 6, 2006 Primitive erythropoiesis from mesodermal precursors expressing VE-cadherin, PECAM-1, Tie2, endoglin and CD34 in the mouse embryo Masatsugu Ema*, Tomomasa Yokomizo, Asami Wakamatsu, Tsumoru Terunuma, Masayuki Yamamoto, and Satoru Takahashi Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan Center for TARA, The University of Tsukuba, Japan * Corresponding author; email: masaema{at}md.tsukuba.ac.jp. Vascular endothelial (VE) cadherin, PECAM-1 (Platelet Endothelial cell adhesion molecule-1), Tie2, CD34 and Endoglin are established markers for adult and embryonic endothelial cells (ECs). Here, we report that the expression of these EC markers is initiated in the extraembryonic region at the late-streak stage (nominal stage E6.75). Immunohistochemical analysis shows that EC marker-positive cells arise in a subset of Flk1 (VEGF-R2) mesodermal cells. In contrast, GATA1, a marker for primitive erythropoietic progenitors, is expressed in a more restricted subset of Flk1-positive cells. Using flow cytometry, we observed that the GATA1-positive cell population existed as a subset of the EC marker-positive cell. Consistent with this notion, we showed that primitive erythropoietic progenitors are enriched in PECAM-1- and Tie2-positive cells using the primitive hematopoietic colony assay. These results suggest that primitive hematopoietic cells arise from EC marker-positive cells. Thus, VE-cadherin, PECAM-1, CD34, Endoglin and Tie2 are not only expressed in adult and embryonic ECs, but also in extraembryonic Flk1-positive cells during gastrulation. The latter cell population includes progenitors giving rise to primitive hematopoietic cells, suggesting that both primitive and definitive hematopoietic cells in the mouse embryo arise from EC marker-positive cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Yokomizo, K. Hasegawa, H. Ishitobi, M. Osato, M. Ema, Y. Ito, M. Yamamoto, and S. Takahashi Runx1 is involved in primitive erythropoiesis in the mouse Blood, April 15, 2008; 111(8): 4075 - 4080. [Abstract] [Full Text] [PDF] R. C. R. Perlingeiro Endoglin is required for hemangioblast and early hematopoietic development Development, August 15, 2007; 134(16): 3041 - 3048. [Abstract] [Full Text] [PDF]

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