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Blood, 15 September 2006, Vol. 108, No. 6, pp. 2121-2123.
Prepublished online as a Blood First Edition Paper on May 30, 2006; DOI 10.1182/blood-2006-03-013599.
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Submitted March 31, 2006
Accepted April 21, 2006
Short term repopulating cells with myeloid potential in
human mobilized peripheral blood do not have a side
population (SP) phenotype
Marlene Fischer, Manfred Schmidt, Silke Klingenberg, Connie J Eaves, Christof von Kalle*, and Hanno Glimm
Department of Internal Medicine I,Faculty of Biology, Albert-Ludwigs-University Freiburg, Germany
Terry Fox Laboratory, British Columbia Cancer Agency and Department of Medical Genetics, UBC,Canada
National Center for Tumor Diseases, German Cancer Research Center, Heidelberg, Germany
* Corresponding author; email: christof.kalle{at}nct-heidelberg.de.
Clinical use of purified hematopoietic stem cells in
myeloablated patients requires co-transplantation of
short-term repopulating cells (STRCs) to ensure timely
count recovery. Here we investigated the flow
fluorescence-based side population (SP) phenotype of
mobilized human peripheral blood (mPB) cells that
rapidly repopulate the highly permissive NOD/SCID-
 2microglobulin-/- mouse. No SP cells from this source
regenerated detectable progeny in these mice before 8
weeks, although by 12 weeks human B-lymphoid cells were
seen in some recipients of SP mPB cells. All myeloid
reconstituting activity, including that seen within 3
weeks after transplant was associated with the non-SP
fraction. Isolation of SP cells depletes human mPB of
the rapid myeloid reconstitution capacity provided by
myeloid-restricted STRC which are vital for early
hematologic recovery in clinical transplant recipients.
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