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Blood, 15 November 2006, Vol. 108, No. 10, pp. 3397-3405. Prepublished online as a Blood First Edition Paper on July 18, 2006; DOI 10.1182/blood-2006-04-014779. This Article Full Text (PDF) All Versions of this Article: blood-2006-04-014779v1 108/10/3397    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nasirikenari, M. Articles by Lau, J. T Search for Related Content PubMed PubMed Citation Articles by Nasirikenari, M. Articles by Lau, J. T Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 3, 2006 Accepted July 3, 2006 Altered granulopoietic profile and exaggerated acute neutrophilic inflammation in mice with targeted deficiency in the sialyltransferase, ST6Gal I Mehrab Nasirikenari, Brahm H Segal, Julie R Ostberg, Ashlee Urbasic, and Joseph T Lau* Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, NY, USA Departments of Medicine and Immunology, Roswell Park Cancer Institute, NY, USA Department of Immunology, Roswell Park Cancer Institute, NY, USA Department of Pathobiology, U. of Illinois, Urbana, IL, USA * Corresponding author; email: joseph.lau{at}roswellpark.org. Elevation of serum sialic acid and the ST6Gal-1 sialyltransferase are part of the hepatic systemic inflammatory response, but the contribution of ST6Gal-1 has remained unclear. ST6Gal-1 elevation is mediated by P1, one of six promoters regulating the ST6Gal-1 gene. We report that the P1-ablated mouse, Siat1P1, and a globally ST6Gal-1 deficient mouse had significantly increased peritoneal leukocytosis after intraperitoneal challenge with thioglycollate. Exaggerated peritonitis was accompanied by only a modest increase in neutrophil viability, and transferred bone marrow-derived neutrophils from Siat1P1 mice migrated to the peritoneum of recipients with normal efficiency following thioglycollate challenge. Siat1P1 mice exhibited three-fold greater neutrophilia by thioglycollate, greater pool of epinephrine-releasable marginated neutrophils, greater sensitivity to G-CSF, elevated bone marrow CFU-G and proliferative stage myeloid cells, and a more robust recovery from cyclophosphoramide-induced myelosuppression. Bone marrow leukocytes from Siat1P1 are indistinguishable from wild-type mice in 2,6-sialylation as revealed by the Sambucus nigra lectin, and in the expression of total ST6Gal-1 mRNA. Together, our study demonstrates a role for ST6Gal-1, possibly from extramedullary sources (e.g. that produced in liver), in regulating inflammation, circulating neutrophil homeostasis, and in replenishment of granulocyte numbers. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Wan, J. M. C. Coppens, C. G. van Helden-Meeuwsen, P. J. M. Leenen, N. van Rooijen, N. A. Khan, R. C. M. Kiekens, R. Benner, and M. A. Versnel Chorionic gonadotropin alleviates thioglycollate-induced peritonitis by affecting macrophage function J. Leukoc. Biol., August 1, 2009; 86(2): 361 - 370. [Abstract] [Full Text] [PDF] F. Guillerme-Bosselut, L. Forestier, C. Jayat-Vignoles, J.-L. Vilotte, I. Popa, J. Portoukalian, A. Le Dur, H. Laude, R. Julien, and P.-F. Gallet Glycosylation-related gene expression profiling in the brain and spleen of scrapie-affected mouse Glycobiology, August 1, 2009; 19(8): 879 - 889. [Abstract] [Full Text] [PDF] N. Malagolini, M. Chiricolo, M. Marini, and F. Dall'Olio Exposure of {alpha}2,6-sialylated lactosaminic chains marks apoptotic and necrotic death in different cell types Glycobiology, February 1, 2009; 19(2): 172 - 181. [Abstract] [Full Text] [PDF] D. D. Marathe, E. V. Chandrasekaran, J. T. Y. Lau, K. L. Matta, and S. Neelamegham Systems-level studies of glycosyltransferase gene expression and enzyme activity that are associated with the selectin binding function of human leukocytes FASEB J, December 1, 2008; 22(12): 4154 - 4167. [Abstract] [Full Text] [PDF] J. H Marino, C. Tan, B. Davis, E.-S. Han, M. Hickey, R. Naukam, A. Taylor, K. S Miller, C J. Van De Wiele, and T K. Teague Disruption of thymopoiesis in ST6Gal I-deficient mice Glycobiology, September 1, 2008; 18(9): 719 - 726. [Abstract] [Full Text] [PDF]

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