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Blood, 15 January 2007, Vol. 109, No. 2, pp. 643-652. Prepublished online as a Blood First Edition Paper on September 12, 2006; DOI 10.1182/blood-2006-04-016840. This Article Full Text (PDF) All Versions of this Article: blood-2006-04-016840v1 109/2/643    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gogolak, P. Articles by Rajnavolgyi, E. Search for Related Content PubMed PubMed Citation Articles by Gogolak, P. Articles by Rajnavolgyi, E. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 26, 2006 Accepted August 20, 2006 Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPAR Peter Gogolak, Bence Rethi, Istvan Szatmari, Arpad Lanyi, Balazs Dezso, Laszlo Nagy, and Eva Rajnavolgyi* Institute of Immunology, University of Debrecen, Debrecen, Hungary Institute of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary Department of Pathology, University of Debrecen, Debrecen, Hungary Department of Immunology, University of Debrecen, Debrecen, Hungary * Corresponding author; email: evaraj{at}jaguar.unideb.hu. Accumulating data have shown that the microenvironment of dendritic cells modulates subtype differentiation and CD1 expression, but the mechanisms by which exogenous factors confer these effects are poorly understood. Here we describe the dependence of monocyte-derived CD1a- dendritic cell (moDC) development on lipids associated with the expression of peroxisome proliferator-activated receptor-gamma (PPAR). We also show the consecutive differentiation of immature CD1a-PPAR+ moDCs to CD1a+PPAR- cells limited by serum lipoproteins and terminated by pro-inflammatory cytokines. Immature CD1a- moDCs possess higher internalizing capacity than CD1a+ cells while both activated subtypes have similar migratory potential but differ in their cytokine and chemokine profiles that translates to distinct T-lymphocyte polarizing capacities. CD1a+ moDCs stand out by their capability to secrete high amounts of IL-12p70 and CCL1. As lipoproteins skew moDC differentiation toward the generation of CD1a-PPAR+ cells and inhibit the development of CD1a+PPAR- cells we suggest that the uptake of lipids results in endogenous PPAR agonists that induce a cascade of gene transcription coordinating lipid metabolism, the expression of lipid presenting CD1 molecules, subtype dichotomy and function. The presence of CD1a-PPAR+ and CD1a+PPAR- DCs in lymph nodes and in pulmonary Langerhans cell histiocytosis confirms the functional relevance of these DC subsets in vivo. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Smed-Sorensen, M. Moll, T.-Y. Cheng, K. Lore, A.-C. Norlin, L. Perbeck, D. B. Moody, A.-L. Spetz, and J. K. Sandberg IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via Fc{gamma}RIIa Blood, May 15, 2008; 111(10): 5037 - 5046. [Abstract] [Full Text] [PDF] I. Szatmari, D. Torocsik, M. Agostini, T. Nagy, M. Gurnell, E. Barta, K. Chatterjee, and L. Nagy PPAR{gamma} regulates the function of human dendritic cells primarily by altering lipid metabolism Blood, November 1, 2007; 110(9): 3271 - 3280. [Abstract] [Full Text] [PDF]

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