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Blood, 15 November 2006, Vol. 108, No. 10, pp. 3360-3362. Prepublished online as a Blood First Edition Paper on August 3, 2006; DOI 10.1182/blood-2006-04-018036. This Article Full Text (PDF) Supplemental Table All Versions of this Article: blood-2006-04-018036v1 108/10/3360    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pastos, K. M Articles by Sola-Visner, M. C Search for Related Content PubMed PubMed Citation Articles by Pastos, K. M Articles by Sola-Visner, M. C Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 3, 2006 Accepted July 10, 2006 Differential effects of recombinant thrombopoietin and bone marrow stromal conditioned media on neonatal vs. adult megakaryocytes Karen M Pastos, William B Slayton, Lisa M Rimsza, Linda Young, and Martha C Sola-Visner* Shands Cancer Center and the Departments, University of Florida, FL, USA Department of Pediatrics, University of Florida, FL, USA Department of Pathology, University of Arizona, Tucson, AZ, USA Department of Biostatistics, University of Florida, FL, USA * Corresponding author; email: martha.sola-visner{at}drexelmed.edu. Umbilical cord blood (CB) is a valuable source of stem cells for transplantation, but CB transplants are frequently complicated by delayed platelet engraftment. The reasons underlying this are unclear. We hypothesized that CB- and peripheral blood (PB)-derived megakaryocytes (MKs) respond differently to the adult hematopoietic microenvironment and to thrombopoietin (Tpo). To test this, we cultured CB- and PB-CD34+ cells in adult bone marrow stromal conditioned media (CM) or unconditioned media (UCM) with increasing concentrations of recombinant Tpo, and compared the effects of these conditions on CB- vs. PB-MKs. PB-MKs reached highest ploidy in response to UCM + 100 ng/ml rTpo, and the addition of CM inhibited their maturation. In contrast, CB-MKs reached highest ploidy in CM without rTpo, and high rTpo concentrations (>0.1 ng/ml) inhibited their maturation. This is the first evidence that human neonatal and adult MKs have substantially different biological responses to Tpo and potentially to other cytokines. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Dominici, V. Rasini, R. Bussolari, X. Chen, T. J. Hofmann, C. Spano, D. Bernabei, E. Veronesi, F. Bertoni, P. Paolucci, et al. Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation Blood, September 10, 2009; 114(11): 2333 - 2343. [Abstract] [Full Text] [PDF] M. A. Saxonhouse and M. C. Sola-Visner Thrombocytopenia in the Neonatal Intensive Care Unit NeoReviews, September 1, 2009; 10(9): e435 - e445. [Abstract] [Full Text] [PDF] R. Flaumenhaft, J. R. Dilks, J. Richardson, E. Alden, S. R. Patel-Hett, E. Battinelli, G. L. Klement, M. Sola-Visner, and J. E. Italiano Jr Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles Blood, January 29, 2009; 113(5): 1112 - 1121. [Abstract] [Full Text] [PDF]

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