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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2521-2528. Prepublished online as a Blood First Edition Paper on November 14, 2006; DOI 10.1182/blood-2006-04-018085. This Article Full Text (PDF) All Versions of this Article: blood-2006-04-018085v1 109/6/2521    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lindsley, R. C. Articles by Allman, D. Search for Related Content PubMed PubMed Citation Articles by Lindsley, R. C. Articles by Allman, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 19, 2006 Accepted October 23, 2006 Generation of peripheral B cells occurs via two spatially and temporally distinct pathways Robert Coleman Lindsley, Matthew Thomas, Bhaskar Srivastava, and David Allman* Dept of Pathology & Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA * Corresponding author; email: dallman{at}mail.med.upenn.edu. We have identified a population of newly formed bone marrow (BM) B cells that shares multiple characteristics with late transitional B cells in the spleen. Both late splenic transitional B cells and cells within this uncharacterized BM population expressed the cell surface phenotype AA4+ CD23+, yet the developmental kinetics and the renewal rate of AA4+ CD23+ BM B cells mirrored recently formed BM B cells. Further, unlike the least mature B cells in the BM and spleen, AA4+ CD23+ BM B cells expressed the homing receptor CD62L, were dependent on the anti-apoptotic cytokine receptor BR3 and the tec family kinase Btk, and proliferated in response to IL-4 plus CD40 stimulation. Finally, frequencies of light chain+ B cells declined among AA4+ CD23+ B cells in both the BM and spleen, suggesting that V-gene selection events correlate with CD23 expression in both compartments. These observations indicate that the first step in B cell maturation occurs in both the BM and the periphery, and suggest that recently formed B cells exit the BM as a heterogeneous pool of immature and semi-mature B cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Palanichamy, J. Barnard, B. Zheng, T. Owen, T. Quach, C. Wei, R. J. Looney, I. Sanz, and J. H. Anolik Novel Human Transitional B Cell Populations Revealed by B Cell Depletion Therapy J. Immunol., May 15, 2009; 182(10): 5982 - 5993. [Abstract] [Full Text] [PDF] A. J. Gross, J. R. Lyandres, A. K. Panigrahi, E. T. L. Prak, and A. L. DeFranco Developmental Acquisition of the Lyn-CD22-SHP-1 Inhibitory Pathway Promotes B Cell Tolerance J. Immunol., May 1, 2009; 182(9): 5382 - 5392. [Abstract] [Full Text] [PDF] E. Claudio, S. Saret, H. Wang, and U. Siebenlist Cell-Autonomous Role for NF-{kappa}B in Immature Bone Marrow B Cells J. Immunol., March 15, 2009; 182(6): 3406 - 3413. [Abstract] [Full Text] [PDF] S. Alter-Wolf, B. B. Blomberg, and R. L. Riley Deviation of the B Cell Pathway in Senescent Mice Is Associated with Reduced Surrogate Light Chain Expression and Altered Immature B Cell Generation, Phenotype, and Light Chain Expression J. Immunol., January 1, 2009; 182(1): 138 - 147. [Abstract] [Full Text] [PDF] G. Zekavat, S. Y. Rostami, A. Badkerhanian, R. F. Parsons, B. Koeberlein, M. Yu, C. D. Ward, T.-S. Migone, L. Yu, G. S. Eisenbarth, et al. In Vivo BLyS/BAFF Neutralization Ameliorates Islet-Directed Autoimmunity in Nonobese Diabetic Mice J. Immunol., December 1, 2008; 181(11): 8133 - 8144. [Abstract] [Full Text] [PDF] J. B. Carey, C. S. Moffatt-Blue, L. C. Watson, A. L. Gavin, and A. J. Feeney Repertoire-based selection into the marginal zone compartment during B cell development J. Exp. Med., September 1, 2008; 205(9): 2043 - 2052. [Abstract] [Full Text] [PDF]

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