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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2346-2355. Prepublished online as a Blood First Edition Paper on November 21, 2006; DOI 10.1182/blood-2006-04-019034. This Article Full Text (PDF) Supplemental Methods and Table Supplemental Figures Supplemental Figures All Versions of this Article: blood-2006-04-019034v1 109/6/2346    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dorn, T. Articles by Brakebusch, C. Search for Related Content PubMed PubMed Citation Articles by Dorn, T. Articles by Brakebusch, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 25, 2006 Accepted November 2, 2006 RhoH is important for positive thymocyte selection and T cell receptor signaling Tatjana Dorn, Ursula Kuhn, Gerd Bungartz, Sebastian Stiller, Martina Bauer, Joachim Ellwart, Thorsten Peters, Karin Scharffetter-Kochanek, Monika Semmrich, Melanie Laschinger, Bernhard Holzmann, Wolfgang E.F. Klinkert, Per Thor Straten, Tania Kollgaard, Michael Sixt, and Cord Brakebusch* Heisenberg Group "Regulation of Cytoskeletal Organization", Max Planck Institute of Biochemistry, Martinsried, Germany Dept of Molecular Medicine, Max Planck Institute of Biochemistry, Martinsried, Germany GSF, Institute of Molecular Immunology, Munchen, Germany Clinic for Dermatology & Allergology, University of Ulm, Ulm, Germany Dept of Surgery, Technische Universitaet Muenchen, Munchen, Germany Dept of Neuroimmunology, Max Planck Institute of Neurobiology, Martinsried, Germany Dept of Hematology, Center for Cancer Immunotherapy (CCIT), University Hospital Herlev, Denmark Dept of Molecular Pathology, University of Copenhagen, Copenhagen, Denmark * Corresponding author; email: cord{at}pai.ku.dk. RhoH is a small GTPase expressed only in the hematopoietic system. Using mice with a targeted disruption of the RhoH gene we demonstrate that RhoH is crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Furthermore, differentiation and expansion of DN3 and DN4 thymocytes in vitro is severely impaired. These defects correspond to a defective TCR signaling. While RhoH is not required for TCR induced activation of ZAP70 and ZAP70 mediated activation of p38, it is crucial for tyrosine phosphorylation of LAT, PLC1 and Vav1 and for the activation of Erk and calcium influx. These data suggest that RhoH is important for pre-TCR and TCR signaling by allowing the efficient interaction of ZAP70 with the LAT signalosome, thus regulating thymocyte development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M.-H. Fortier, E. Caron, M.-P. Hardy, G. Voisin, S. Lemieux, C. Perreault, and P. Thibault The MHC class I peptide repertoire is molded by the transcriptome J. Exp. Med., March 17, 2008; 205(3): 595 - 610. [Abstract] [Full Text] [PDF]

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