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Blood, 1 January 2007, Vol. 109, No. 1, pp. 85-92. Prepublished online as a Blood First Edition Paper on September 5, 2006; DOI 10.1182/blood-2006-05-020289. This Article Full Text (PDF) Supplemental Table All Versions of this Article: blood-2006-05-020289v1 109/1/85    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Iwata, M. Articles by Torok-Storb, B. S Search for Related Content PubMed PubMed Citation Articles by Iwata, M. Articles by Torok-Storb, B. S Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 12, 2006 Accepted August 12, 2006 Reduced expression of inducible gelatinase B/matrix metalloproteinase-9 in monocytes from patients with myelodysplastic syndrome: Correlation of inducible levels with the percentage of cytogenetically-marked cells and with marrow cellularity Mineo Iwata, Manoj Pillai, Aravind Ramakrishnan, Robert C. Hackman, H Joachim Deeg, Ghislain M.M. Opdenakker, and Beverly S Torok-Storb* Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA Fred Hutchinson Cancer Research Center Fred Hutchinson Cancer Research Center and University of Washington, Seattle, WA Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Belgium * Corresponding author; email: btorokst{at}fhcrc.org. Regulatory molecules produced by stromal cells are often membrane-bound until cleaved by matrix metalloproteinases (MMP); cleavage can serve to either activate or inactivate regulatory functions. We report here that marrow stromal cells induce the expression of MMP-9 in monocytes. Induction was contact independent and could be reproduced with recombinant MCP-1/CCL2, whereas IL-6, M-CSF, G-CSF, GM CSF, IL-8/CXCL8, SDF-1/CXCL12, and MGSA/CXCL1 did not have this effect. Stroma-induced levels of MMP-9 in the monocyte population from healthy donors were relatively consistent, whereas induced levels varied significantly (P<0.001) in the CD14+ population from 27 patients with myelodysplastic syndrome (MDS). In patients with a clonal chromosomal marker, the level of inducible MMP-9 expression in the monocyte population was inversely correlated with the percentage of marker-positive cells (n=11, P=0.01), suggesting that the ability to induce MMP-9 may be compromised in clonally-derived monocytes. The inducible levels of MMP-9 were also inversely correlated with marrow cellularity observed in biopsies from MDS patients (P=0.0006). We conclude that monocytes can express MMP-9 in response to stromal factors and that this response may be significantly decreased in MDS-derived monocytes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. E. Cramer, S. Wagner, B. Li, J. Liu, R. Hansen, R. Reca, W. Wu, E. Z. Surma, D. A. Laber, M. Z. Ratajczak, et al. Mobilization of Hematopoietic Progenitor Cells by Yeast-Derived {beta}-Glucan Requires Activation of Matrix Metalloproteinase-9 Stem Cells, May 1, 2008; 26(5): 1231 - 1240. [Abstract] [Full Text] [PDF]

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