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Blood, 1 October 2006, Vol. 108, No. 7, pp. 2384-2391. Prepublished online as a Blood First Edition Paper on June 6, 2006; DOI 10.1182/blood-2006-05-020602. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: blood-2006-05-020602v1 108/7/2384    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rankin, C. T Articles by Bonvini, E. Search for Related Content PubMed PubMed Citation Articles by Rankin, C. T Articles by Bonvini, E. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 12, 2006 Accepted May 23, 2006 CD32B, the human inhibitory Fc- receptor IIB, as a target for monoclonal antibody therapy of B-cell lymphoma Christopher T Rankin, Maria-Concetta Veri, Sergey Gorlatov, Nadine Tuaillon, Steve Burke, Ling Huang, David Inzunza, Hua Li, Shannon Thomas, Syd Johnson, Jeffrey Stavenhagen, Scott Koenig, and Ezio Bonvini* MacroGenics Inc., Rockville, MD, USA * Corresponding author; email: bonvinie{at}macrogenics.com. Human CD32B (FcRIIB), the low affinity inhibitory receptor for IgG, is the predominant Fc- receptor (FcR) present on B-cells. Immunohistochemical and expression studies have identified CD32B expression in a variety of B-cell malignancies, suggesting that CD32B is a potential immunotherapeutic target for B-cell malignancies. A high-affinity monoclonal antibody (mAb 2B6), from a novel panel of anti-human CD32B-specific mAbs was chimerized (ch2B6) and humanized (hu2B6-3.5). Both ch2B6 and hu2B6-3.5 were capable of directing cytotoxicity by peripheral blood mononuclear cells (PBMC) and monocyte-derived macrophages against B- lymphoma lines in vitro. In a human B-cell lymphoma mouse xenograft model, administration of ch2B6 or hu2B6- 3.5 reduced tumor growth rate and improved tumor-free survival. Both the in vitro and in vivo activities of 2B6 required an intact Fc, suggesting an FcR-mediated mechanism of action. These data support the hypothesis that CD32B is a viable target for monoclonal antibody treatment of B-cell lymphoproliferative disorders. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. C. Willcocks, P. A. Lyons, M. R. Clatworthy, J. I. Robinson, W. Yang, S. A. Newland, V. Plagnol, N. N. McGovern, A. M. Condliffe, E. R. Chilvers, et al. Copy number of FCGR3B, which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake J. Exp. Med., July 7, 2008; 205(7): 1573 - 1582. [Abstract] [Full Text] [PDF] P. Zhou, R. L. Comenzo, A. B. Olshen, E. Bonvini, S. Koenig, P. G. Maslak, M. Fleisher, J. Hoffman, S. Jhanwar, J. W. Young, et al. CD32B is highly expressed on clonal plasma cells from patients with systemic light-chain amyloidosis and provides a target for monoclonal antibody-based therapy Blood, April 1, 2008; 111(7): 3403 - 3406. [Abstract] [Full Text] [PDF] J. B. Stavenhagen, S. Gorlatov, N. Tuaillon, C. T. Rankin, H. Li, S. Burke, L. Huang, S. Johnson, E. Bonvini, and S. Koenig Fc Optimization of Therapeutic Antibodies Enhances Their Ability to Kill Tumor Cells In vitro and Controls Tumor Expansion In vivo via Low-Affinity Activating Fc{gamma} Receptors Cancer Res., September 15, 2007; 67(18): 8882 - 8890. [Abstract] [Full Text] [PDF]

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