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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2365-2372. Prepublished online as a Blood First Edition Paper on November 9, 2006; DOI 10.1182/blood-2006-05-022640. This Article Full Text (PDF) All Versions of this Article: blood-2006-05-022640v1 109/6/2365    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chute, J. P Articles by Chao, N. J Search for Related Content PubMed PubMed Citation Articles by Chute, J. P Articles by Chao, N. J Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 19, 2006 Accepted October 29, 2006 Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice John P Chute*, Garrett G Muramoto, Alice B Salter, Sarah K Meadows, Dennis Rickman, Benny Chen, Heather A Himburg, and Nelson J Chao Division of Cellular Therapy, Department of Medicine, Duke University Medical Center, Durham, NC Department of Neurobiology, Duke University Medical Center, Durham, NC * Corresponding author; email: john.chute{at}duke.edu. Flk-1+ endothelial progenitors contribute critically to the definitive onset of hematopoiesis during embryogenesis. Recent studies have suggested that adult sources of endothelial cells also possess hematopoietic activity. In this study we sought to determine whether transplantation of primary vascular endothelial cells (ECs) could enhance the hematopoietic recovery and survival of irradiated mice. C57Bl6 mice were exposed to sublethal and lethal doses of irradiation and subsequently transplanted with either primary murine brain-derived ECs (MBECs) or fetal blood-derived ECs (FBECs). Mice transplanted with MBECs alone demonstrated accelerated BM cellular recovery, radioprotection of BM c-kit+sca-1- lin- progenitors and enhanced regeneration of c-kit+sca-1+lin- (KSL) stem/progenitor cells following irradiation compared to controls. MBEC transplantation also facilitated the recovery of circulating white blood cells and platelet counts following radiation exposure. Remarkably, 57% of mice transplanted with MBECs alone survived long-term following 1050 cGy exposure, which was 100% lethal in control mice. FBEC transplantation was also associated with increased survival compared to controls, although these mice did not survive long-term. These data suggest that reestablishment of endothelial cell activity can improve the hematopoietic recovery and survival of irradiated mice. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Endothelial cell–based therapy for hematopoiesis Dan G. Duda Blood 2007 109: 2272-2273. [Full Text] [PDF] This article has been cited by other articles: J. S. GREENBERGER Radioprotection In Vivo, March 1, 2009; 23(2): 323 - 336. [Abstract] [Full Text] [PDF] A. B. Salter, S. K. Meadows, G. G. Muramoto, H. Himburg, P. Doan, P. Daher, L. Russell, B. Chen, N. J. Chao, and J. P. Chute Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo Blood, February 26, 2009; 113(9): 2104 - 2107. [Abstract] [Full Text] [PDF]

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