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Blood, 1 March 2007, Vol. 109, No. 5, pp. 1825-1833. Prepublished online as a Blood First Edition Paper on October 31, 2006; DOI 10.1182/blood-2006-05-023028. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-05-023028v1 109/5/1825    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Forde, S. P Articles by Watt, S. M Search for Related Content PubMed PubMed Citation Articles by Forde, S. P Articles by Watt, S. M Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 12, 2006 Accepted October 23, 2006 Endolyn (CD164) Modulates the CXCL12-Mediated Migration of Umbilical Cord Blood CD133+ Cells Sinead P Forde, Britt Jorgensen Tye, Sarah E Newey, Maria Roubelakis, Jon Smythe, Colin P McGuckin, Ruth Pettengell, and Suzanne M Watt* Stem Cell Laboratory, National Blood Service, National Blood & Transplant Authority, Oxford, United Kingdom Nuffield Dept of Clinical Laboratory Sciences, University of Oxford, Oxford, United Kingdom Haematological Sciences & the International Centre for Life, University of Newcastle upon Tyne, Newcastle, United Kingdom St George's, University of London, United Kingdom * Corresponding author; email: suzanne.watt{at}nbs.nhs.uk. Hematopoietic stem/progenitor cell (HSC/HPC) homing to specific microenvironmental niches involve interactions between multiple receptor-ligand pairs. Although CXCL12/CXCR4 play a central role in these events, CXCR4 regulators that provide the specificity for such cells to lodge and be retained in particular niches are poorly defined. Here, we provide evidence that the sialomucin, endolyn (CD164), an adhesion receptor that regulates CD34+ cell adhesion to bone marrow stroma and recruitment of CD34+CD38lo/- cells into cycle, associates with CXCR4. The class II 103B2 monoclonal antibody which binds the CD164 N-linked glycan-dependent epitope or CD164 knockdown by RNA interference significantly inhibits the migration of CD133+ HPC towards CXCL12 in vitro. On presentation of CXCL12 on fibronectin, CD164 associates with CXCR4, an interaction that temporally follows the association of CXCR4 with the integrins, VLA-4 and VLA-5. This coincides with signaling through the CXCR4 receptor via PKC- and Akt, which were disrupted on loss of CD164, while MAPK signaling was unaffected. We therefore demonstrate a novel association between three distinct families of cell surface receptors that regulate cell migratory responses and identify a new role for CD164. We propose that this lends specificity to the homing and lodgement of these cells within the bone marrow niche. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. S. Nielsen and K. M. McNagny Novel functions of the CD34 family J. Cell Sci., November 15, 2008; 121(22): 3683 - 3692. [Abstract] [Full Text] [PDF] G.-U. Bae, U. Gaio, Y.-J. Yang, H.-J. Lee, J.-S. Kang, and R. S. Krauss Regulation of Myoblast Motility and Fusion by the CXCR4-associated Sialomucin, CD164 J. Biol. Chem., March 28, 2008; 283(13): 8301 - 8309. [Abstract] [Full Text] [PDF]

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