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Blood, 15 December 2006, Vol. 108, No. 13, pp. 3976-3978. Prepublished online as a Blood First Edition Paper on August 22, 2006; DOI 10.1182/blood-2006-05-024075. This Article Full Text (PDF) Supplemental Video All Versions of this Article: blood-2006-05-024075v1 108/13/3976    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Renshaw, S. A Articles by Whyte, M. K Search for Related Content PubMed PubMed Citation Articles by Renshaw, S. A Articles by Whyte, M. K Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 22, 2006 Accepted August 7, 2006 A transgenic zebrafish model of neutrophilic inflammation Stephen A Renshaw*, Catherine A Loynes, Daniel M Trushell, Stone Elworthy, Philip W Ingham, and Moira K Whyte MRC Centre Development for Developmental and Biomedical Genetics, University of Sheffield, UK Academic Unit of Respiratory Medicine,School of Medicine & Biomedical ScienceUniversity of Sheffield * Corresponding author; email: s.a.renshaw{at}sheffield.ac.uk. We have established an in vivo model for the genetic analysis of the inflammatory response by generating a transgenic zebrafish line which expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced following transection of the tail of zebrafish larvae, and that this inflammation subsequently resolves over a similar timecourse to mammalian systems. Quantitative data can be generated from this model, by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally-induced inflammation can be inhibited by the addition of a pan-caspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Fluorescent neutrophils throw the spotlight on inflammation Graham J. Lieschke Blood 2006 108: 3961-3962. [Full Text] [PDF] This article has been cited by other articles: A. Lin, J. A. Loughman, B. H. Zinselmeyer, M. J. Miller, and M. G. 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