SEARCH:   Advanced

Blood, 1 November 2006, Vol. 108, No. 9, pp. 3168-3175. Prepublished online as a Blood First Edition Paper on July 6, 2006; DOI 10.1182/blood-2006-05-024406. This Article Full Text (PDF) All Versions of this Article: blood-2006-05-024406v1 108/9/3168    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yadav, M. Articles by Schorey, J. S Search for Related Content PubMed PubMed Citation Articles by Yadav, M. Articles by Schorey, J. S Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 27, 2005 Accepted June 24, 2006 The -glucan receptor Dectin-1 functions together with TLR2 to mediated macrophage activation by mycobacteria Mahesh Yadav and Jeffrey S Schorey* Department of Biological Sciences, Center for Tropical Disease Research, University of Notre Dame * Corresponding author; email: schorey.1{at}nd.edu. Pattern recognition receptors (PRRs) play an essential role in a macrophage’s response to mycobacterial infections. However, how these receptors work in concert to promote this macrophage response remains unclear. In this study, we used bone marrow-derived macrophages isolated from Mannose Receptor (MR), Complement Receptor 3 (CR3), MyD88, Toll-like receptor 4 (TLR4) and TLR2 knockout mice to examine the significance of these receptors in mediating a macrophage's response to a mycobacterial infection. We determined that MAPK activation and TNF- production in macrophage infected with M. avium or M. smegmatis is dependent on MyD88 and TLR2 but not TLR4, MR or CR3. Interestingly, the TLR2 mediated production of TNF- by macrophages infected with M. smegmatis required the -glucan receptor Dectin-1. A similar requirement for Dectin-1 in TNF- production was observed for macrophages infected with M. bovis BCG, M. phlei, M. avium 2151-rough and M. tuberculosis H37Ra. The limited production of TNF- by virulent M. avium 724 and M. tuberculosis H37Rv was not dependent on Dectin-1. Furthermore, Dectin-1 facilitated IL-6, RANTES and G-CSF production by mycobacteria-infected macrophages. These are the first results to establish a significant role for Dectin-1, in cooperation with TLR2, to activate a macrophage's pro-inflammatory response to a mycobacterial infection. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. D. Mossalayi, I. Vouldoukis, M. Mamani-Matsuda, T. Kauss, J. Guillon, J. Maugein, D. Moynet, J. Rambert, V. Desplat, D. Mazier, et al. CD23 Mediates Antimycobacterial Activity of Human Macrophages Infect. Immun., December 1, 2009; 77(12): 5537 - 5542. [Abstract] [Full Text] [PDF] J. Geurtsen, S. Chedammi, J. Mesters, M. Cot, N. N. Driessen, T. Sambou, R. Kakutani, R. Ummels, J. Maaskant, H. Takata, et al. Identification of Mycobacterial {alpha}-Glucan As a Novel Ligand for DC-SIGN: Involvement of Mycobacterial Capsular Polysaccharides in Host Immune Modulation J. Immunol., October 15, 2009; 183(8): 5221 - 5231. [Abstract] [Full Text] [PDF] Y.-C. Chang, W.-C. Kao, W.-Y. Wang, W.-Y. Wang, R.-B. Yang, and K. Peck Identification and characterization of oligonucleotides that inhibit Toll-like receptor 2-associated immune responses FASEB J, September 1, 2009; 23(9): 3078 - 3088. [Abstract] [Full Text] [PDF] T. B. M. Hickey, L. M. Thorson, D. P. Speert, M. Daffe, and R. W. Stokes Mycobacterium tuberculosis Cpn60.2 and DnaK Are Located on the Bacterial Surface, Where Cpn60.2 Facilitates Efficient Bacterial Association with Macrophages Infect. Immun., August 1, 2009; 77(8): 3389 - 3401. [Abstract] [Full Text] [PDF] T. Shinohara, T. Pantuso, S. Shinohara, M. Kogiso, Q. N. Myrvik, R. A. Henriksen, and Y. Shibata Persistent Inactivation of Macrophage Cyclooxygenase-2 in Mycobacterial Pulmonary Inflammation Am. J. Respir. Cell Mol. Biol., August 1, 2009; 41(2): 146 - 154. [Abstract] [Full Text] [PDF] V. Driss, F. Legrand, E. Hermann, S. Loiseau, Y. Guerardel, L. Kremer, E. Adam, G. Woerly, D. Dombrowicz, and M. Capron TLR2-dependent eosinophil interactions with mycobacteria: role of {alpha}-defensins Blood, April 2, 2009; 113(14): 3235 - 3244. [Abstract] [Full Text] [PDF] C.-S. Yang, D.-M. Shin, K.-H. Kim, Z.-W. Lee, C.-H. Lee, S. G. Park, Y. S. Bae, and E.-K. Jo NADPH Oxidase 2 Interaction with TLR2 Is Required for Efficient Innate Immune Responses to Mycobacteria via Cathelicidin Expression J. Immunol., March 15, 2009; 182(6): 3696 - 3705. [Abstract] [Full Text] [PDF] S. Xu, J. Huo, K.-G. Lee, T. Kurosaki, and K.-P. Lam Phospholipase C{gamma}2 Is Critical for Dectin-1-mediated Ca2+ Flux and Cytokine Production in Dendritic Cells J. Biol. Chem., March 13, 2009; 284(11): 7038 - 7046. [Abstract] [Full Text] [PDF] K. Werninghaus, A. Babiak, O. Gross, C. Holscher, H. Dietrich, E. M. Agger, J. Mages, A. Mocsai, H. Schoenen, K. Finger, et al. Adjuvanticity of a synthetic cord factor analogue for subunit Mycobacterium tuberculosis vaccination requires FcR{gamma}-Syk-Card9-dependent innate immune activation J. Exp. Med., January 16, 2009; 206(1): 89 - 97. [Abstract] [Full Text] [PDF] S. Karumuthil-Melethil, N. Perez, R. Li, and C. Vasu Induction of Innate Immune Response through TLR2 and Dectin 1 Prevents Type 1 Diabetes J. Immunol., December 15, 2008; 181(12): 8323 - 8334. [Abstract] [Full Text] [PDF] A. Schwegmann and F. Brombacher Host-Directed Drug Targeting of Factors Hijacked by Pathogens Sci. Signal., July 22, 2008; 1(29): re8 - re8. [Abstract] [Full Text] [PDF] N. R. Patel, J. Zhu, S. D. Tachado, J. Zhang, Z. Wan, J. Saukkonen, and H. Koziel HIV Impairs TNF-{alpha} Mediated Macrophage Apoptotic Response to Mycobacterium tuberculosis J. Immunol., November 15, 2007; 179(10): 6973 - 6980. [Abstract] [Full Text] [PDF] A. G. Rothfuchs, A. Bafica, C. G. Feng, J. G. Egen, D. L. Williams, G. D. Brown, and A. Sher Dectin-1 Interaction with Mycobacterium tuberculosis Leads to Enhanced IL-12p40 Production by Splenic Dendritic Cells J. Immunol., September 15, 2007; 179(6): 3463 - 3471. [Abstract] [Full Text] [PDF] S. Chang, A. Dolganiuc, and G. Szabo Toll-like receptors 1 and 6 are involved in TLR2-mediated macrophage activation by hepatitis C virus core and NS3 proteins J. Leukoc. Biol., September 1, 2007; 82(3): 479 - 487. [Abstract] [Full Text] [PDF] K. M. Empey, M. Hollifield, and B. A. Garvy Exogenous Heat-Killed Escherichia coli Improves Alveolar Macrophage Activity and Reduces Pneumocystis carinii Lung Burden in Infant Mice Infect. Immun., July 1, 2007; 75(7): 3382 - 3393. [Abstract] [Full Text] [PDF] A. B. Schromm, J. Howe, A. J. Ulmer, K.-H. Wiesmuller, T. Seyberth, G. Jung, M. Rossle, M. H. J. Koch, T. Gutsmann, and K. Brandenburg Physicochemical and Biological Analysis of Synthetic Bacterial Lipopeptides: VALIDITY OF THE CONCEPT OF ENDOTOXIC CONFORMATION J. Biol. Chem., April 13, 2007; 282(15): 11030 - 11037. [Abstract] [Full Text] [PDF] H. S. Goodridge, R. M. Simmons, and D. M. Underhill Dectin-1 Stimulation by Candida albicans Yeast or Zymosan Triggers NFAT Activation in Macrophages and Dendritic Cells J. Immunol., March 1, 2007; 178(5): 3107 - 3115. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020