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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5191-5198. Prepublished online as a Blood First Edition Paper on March 9, 2007; DOI 10.1182/blood-2006-06-027987. This Article Full Text (PDF) Supplemental Table and Figures All Versions of this Article: blood-2006-06-027987v1 109/12/5191    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wagner, L. A. Articles by Gleich, G. J Search for Related Content PubMed PubMed Citation Articles by Wagner, L. A. Articles by Gleich, G. J Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 8, 2006 Accepted February 28, 2007 EGO, a novel, non-coding RNA gene, regulates eosinophil granule protein transcript expression Lori A. Wagner*, Clarissa J Christensen, Diane M Dunn, Gerald J Spangrude, Ann Georgelas, Linda L Kelley, Michael Sean Esplin, Robert B Weiss, and Gerald J Gleich Department of Dermatology, University of Utah, Salt Lake City, UT, United States Human Genetics, University of Utah, Salt Lake City, UT, United States Department of Medicine, University of Utah, Salt Lake City, UT, United States Departments of Obstetrics and Gynecology, University of Utah, Salt Lake City, UT, United States * Corresponding author; email: lori.wagner{at}hsc.utah.edu. Gene expression profiling of early eosinophil development shows increased transcript levels of pro-inflammatory cytokines, chemokines, transcription factors and a novel gene, EGO (Eosinophil Granule Ontogeny). EGO is nested within an intron of the inositol triphosphate receptor type 1 (ITPR1) gene and is conserved at the nucleotide level; however, the largest open reading frame (ORF) is 86 amino acids. Sucrose density gradients show that EGO is not associated with ribosomes and therefore, is a non-coding RNA (ncRNA). EGO transcript levels rapidly increase following interleukin-5 (IL-5) stimulation of CD34+ hematopoietic progenitors. EGO RNA is also highly expressed in human bone marrow and in mature eosinophils. RNA silencing of EGO results in decreased major basic protein (MBP) and eosinophil derived neurotoxin (EDN) mRNA expression in developing CD34+ hematopoietic progenitors in vitro and in a CD34+ cell line model. Therefore, EGO is a novel ncRNA gene expressed during eosinophil development and is necessary for normal MBP and EDN transcript expression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: X. Zhang, Z. Lian, C. Padden, M. B. Gerstein, J. Rozowsky, M. Snyder, T. R. Gingeras, P. Kapranov, S. M. Weissman, and P. E. Newburger A myelopoiesis-associated regulatory intergenic noncoding RNA transcript within the human HOXA cluster Blood, March 12, 2009; 113(11): 2526 - 2534. [Abstract] [Full Text] [PDF]

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