|
|
Blood, 15 May 2007, Vol. 109, No. 10, pp. 4264-4271.
Prepublished online as a Blood First Edition Paper on February 8, 2007; DOI 10.1182/blood-2006-06-029603.
 Previous Article | Next Article 
Submitted June 20, 2006
Accepted November 19, 2006
c-Kit by interacting with the membrane-bound ligand recruits endothelial progenitor cells to inflamed endothelium
Patrizia Dentelli, Arturo Rosso, Antonina Balsamo, Sofia Colmenares Benedetto, Annarita Zeoli, Marco Pegoraro, Giovanni Camussi, Luigi Pegoraro, and Maria Felice Brizzi*
Dept of Internal Medicine, University of Torino, Torino, Italy
Division of Vascular Surgery, Ospedale Molinette, Torino, Italy
* Corresponding author; email: mariafelice.brizzi{at}unito.it.
We investigated the role of c-Kit and of the membrane-bound ligand (mbKitL) in endothelial progenitor cell (EPC) recruitment by microvascular endothelial cells (EC). We demonstrated that inflammatory activation induced the expression of the mbKitL on EC both in vitro and in vivo, and that recruitment of EPC depended on c-Kit/mbKitL interaction. Depletion of endogenous c-Kit or inhibition of c-Kit enzymatic activity by Imatinib prevented adhesion of EPC to activated EC both in vitro and in vivo, indicating that a functional c-Kit on EPC is essential. We also demonstrate that Akt was the downstream molecule regulating cell adhesion. A potential role of the c-Kit/mbKitL interaction in pathological settings is sustained by the expression of the mbKitL on EC lining intraplaque neovessels. Thus, our results provide new insights into the mechanisms underlying EPC recruitment and the bases for novel strategies to hinder pathological angiogenesis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
Related Article in Blood Online:
-
Inflamed endothelium: an EPC adhesion kit
- Elaine W. Raines
Blood 2007 109: 4118.
[Full Text]
[PDF]
This article has been cited by other articles:
|
|
|
|
 
O. Dotsenko and M. Jahangiri
Endogenous stem cells in patients undergoing coronary artery bypass graft surgery
Eur. J. Cardiothorac. Surg.,
September 1, 2009;
36(3):
563 - 571.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Mukherjee, D. Kamnasaran, A. Balasubramaniam, I. Radovanovic, G. Zadeh, T.-R. Kiehl, and A. Guha
Human Schwannomas Express Activated Platelet-Derived Growth Factor Receptors and c-kit and Are Growth Inhibited by Gleevec (Imatinib Mesylate)
Cancer Res.,
June 15, 2009;
69(12):
5099 - 5107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-M. Kwon, T. Suzuki, A. Kawamoto, M. Ii, M. Eguchi, H. Akimaru, M. Wada, T. Matsumoto, H. Masuda, Y. Nakagawa, et al.
Pivotal Role of Lnk Adaptor Protein in Endothelial Progenitor Cell Biology for Vascular Regeneration
Circ. Res.,
April 24, 2009;
104(8):
969 - 977.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G.M. Molin, N. M.S van den Akker, and M. J. Post
Affirmative Action of Osteopontin on Endothelial Progenitors
Arterioscler Thromb Vasc Biol,
December 1, 2008;
28(12):
2099 - 2100.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Zeoli, P. Dentelli, A. Rosso, G. Togliatto, A. Trombetta, L. Damiano, P. F. di Celle, L. Pegoraro, F. Altruda, and M. F. Brizzi
Interleukin-3 promotes expansion of hemopoietic-derived CD45+ angiogenic cells and their arterial commitment via STAT5 activation
Blood,
July 15, 2008;
112(2):
350 - 361.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
