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 Blood, 15 March 2007, Vol. 109, No. 6, pp. 2356-2364.
Prepublished online as a Blood First Edition Paper on November 9, 2006; DOI 10.1182/blood-2006-06-030031.

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Submitted June 19, 2006
Accepted October 31, 2006

Gfi1b:Green fluorescent protein knock-in mice reveal a dynamic expression pattern of Gfi1b during hematopoiesis that is largely complementary to Gfi1

Lothar Vassen, Taro Okayama, and Tarik Moroy*

Institut fuer Zellbiologie, Germany
Institut de recherches cliniques de Montreal, IRCM, Canada

* Corresponding author; email: moeroey{at}uni-essen.de.

Gfi1b and Gfi1 are 37 and 55 KD transcriptional repressors that share common features such as a 20 amino-acids (aa) N-terminal SNAG domain, an non-conserved intermediary domain and six highly conserved C-terminal zinc-fingers. Both gene loci are under auto- and cross regulatory feedback control. We have generated a reporter mouse strain by inserting the cDNA for green-fluorescent protein (GFP) into the Gfi1b gene locus which allowed us to follow Gfi1b expression during hemato- and lymphopoiesis by measuring green fluorescence. We found highly dynamic expression patterns of Gfi1b in erythroid cells, megakaryocytes and their progenitor cells (MEPS) where Gfi1 is not detected. Vice versa, Gfi1b could not be found in granulocytes, activated macrophages or their granulo-monocytic precursors (GMP) or in mature naive or activated lymphocytes where Gfi1 is expressed suggesting a complementary regulation of both loci during hematopoiesis. However, Gfi1b was found to be up regulated in early stages of B-cell and in a subset of early T-cell development, where Gfi1 is also present, suggesting that cross regulation of both loci exists but is cell type specific.


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