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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5242-5250. Prepublished online as a Blood First Edition Paper on March 8, 2007; DOI 10.1182/blood-2006-06-030619. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-06-030619v1 109/12/5242    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Panes, O. Articles by Mezzano, D. Search for Related Content PubMed PubMed Citation Articles by Panes, O. Articles by Mezzano, D. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 26, 2006 Accepted March 1, 2007 Human platelets synthesize and express functional tissue factor Olga Panes, Valeria Matus, Claudia G. Saez, Teresa Quiroga, Jaime Pereira, and Diego Mezzano* Department of Hematology-Oncology, School of Medicine, Pontificia Universidad Catolica de Chile, Santiago, Chile Department of Clinical Laboratory, School of Medicine, Pontificia Universidad Catolica de Chile, Santiago, Chile * Corresponding author; email: dmezzano{at}med.puc.cl. The source and significance of blood-borne tissue factor (TF) are controversial. TF-mRNA, protein and TF-dependent procoagulant activity (PCA) have been detected in human platelets, but direct evidence of TF synthesis is missing. Non-stimulated, monocyte-free platelets from most individuals expressed TF-mRNA, which was enhanced or induced in all of them after platelet activation. Immunoprecipitation assays revealed TF protein (mainly of Mr ~47kDa with other bands of ~35 and ~60kDa) in non-stimulated platelet membranes, which also increased post-activation. This enhancement was concomitant with TF translocation to the plasma membrane, as demonstrated by immunofluorescence-confocal microscopy and biotinylation of membrane proteins. Platelet PCA, assessed by FXa generation, was induced post-activation and inhibited by 48% and 76% with anti-TF and anti-FVIIa, respectively, but not by intrinsic pathway inhibitors. Platelets incorporated [35S]-methionine into TF proteins of Mr ~47, ~35, and ~60kDa, more intensely after activation. Puromycin, but not actinomycin D or DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) inhibited TF neosynthesis. Thus, human platelets not only assemble the clotting reactions on their membrane but also supply their own TF for thrombin generation in a timely and spatially circumscribed process. These observations simplify, unify and provide a more coherent formulation of the current cell-based model of hemostasis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Platelet tissue factor comes of age Andrew S. Weyrich, Hansjörg Schwertz, and Nigel Mackman Blood 2007 109: 5069-5070. [Full Text] [PDF] This article has been cited by other articles: N. Mackman and M. Taubman Tissue Factor: Past, Present, and Future Arterioscler Thromb Vasc Biol, December 1, 2009; 29(12): 1986 - 1988. [Full Text] [PDF] S. Butenas, T. Orfeo, and K. G. Mann Tissue Factor in Coagulation: Which? Where? When? Arterioscler Thromb Vasc Biol, December 1, 2009; 29(12): 1989 - 1996. [Abstract] [Full Text] [PDF] R. B. Eslam, N. Reiter, A. Kaider, S. Eichinger, I. 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