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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2406-2415. Prepublished online as a Blood First Edition Paper on November 7, 2006; DOI 10.1182/blood-2006-06-031039. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-06-031039v1 109/6/2406    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shinoda, G. Articles by Nakahata, T. Search for Related Content PubMed PubMed Citation Articles by Shinoda, G. Articles by Nakahata, T. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 26, 2006 Accepted October 25, 2006 4-integrin+ endothelium derived from primate embryonic stem cells generates both primitive and definitive hematopoietic cells Gen Shinoda, Katsutsugu Umeda, Toshio Heike, Masato Arai, Akira Niwa, Feng Ma, Hirofumi Suemori, Hong Yuan Luo, David H. K. Chui, Ryuzo Torii, Masabumi Shibuya, Norio Nakatsuji, and Tatsutoshi Nakahata* Graduate School of Medicine, Kyoto University, Japan Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Japan Boston Universal School of Medicine, Boston, MA, United States Shiga University of Medical Science, Japan Institute of Medical Science, University of Tokyo, Japan Institute for Frontier Medical Sciences, Kyoto Univer, Japan * Corresponding author; email: tnakaha{at}kuhp.kyoto-u.ac.jp. The mechanism of commencement of hematopoiesis in blood islands of the yolk sac and the aorta-gonad-mesonephros (AGM) region during primate embryogenesis remains elusive. In this study, we demonstrate that VE-cadherin+CD45- endothelial cells derived from nonhuman primate embryonic stem cells are able to generate primitive and definitive hematopoietic cells sequentially, as revealed by immunostaining of floating erythrocytes and colony-forming assay in cultures. Single bipotential progenitors for hematopoietic and endothelial lineages are included in this endothelial cell population. Furthermore, hemogenic activity of these endothelial cells is observed exclusively in the 4-integrin+ subpopulation and bipotential progenitors are 4-fold enriched in this subpopulation. The kinetics of this hemogenic subpopulation is similar to that of hemogenic endothelial cells previously reported in the yolk sac and the AGM region in vivo, in that they emerge only for a limited time. We suggest that VE-cadherin+CD45-4-integrin+ endothelial cells are involved in primitive as well as definitive hematopoiesis during primate embryogenesis, although VE-cadherin-CD45-4-integrin+ cells are primary sources for primitive hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Wianny, A. Bernat, C. Huissoud, G. Marcy, S. Markossian, V. Cortay, P. Giroud, V. Leviel, H. Kennedy, P. Savatier, et al. Derivation and Cloning of a Novel Rhesus Embryonic Stem Cell Line Stably Expressing Tau-Green Fluorescent Protein Stem Cells, June 1, 2008; 26(6): 1444 - 1453. [Abstract] [Full Text] [PDF]

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