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Blood, 1 August 2007, Vol. 110, No. 3, pp. 860-869. Prepublished online as a Blood First Edition Paper on April 6, 2007; DOI 10.1182/blood-2006-06-031401. This Article Full Text (PDF) All Versions of this Article: blood-2006-06-031401v1 110/3/860    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fukuda, S. Articles by Pelus, L. M. Search for Related Content PubMed PubMed Citation Articles by Fukuda, S. Articles by Pelus, L. M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 26, 2006 Accepted April 3, 2007 The chemokine GRO mobilizes early hematopoietic stem cells characterized by enhanced homing and engraftment Seiji Fukuda, Huimin Bian, Andrew G King, and Louis M. Pelus* Dept Microbiology & Immunology, & Walther Oncology Center, Indiana University School of Medicine, & the Walther Cancer Institute, Indianapolis, IN, United States Microbial, Musculoskeletal and Proliferative Diseases Center, GlaxoSmithKline, Collegeville, PA, United States * Corresponding author; email: lpelus{at}iupui.edu. Mobilized peripheral blood hematopoietic stem cells (PBSC) demonstrate accelerated engraftment compared to bone marrow; however, mechanisms responsible for enhanced engraftment remain unknown. PBSC mobilized by GRO(GRO4/CXCL24) or the combination of GRO4 plus G-CSF restore neutrophil and platelet recovery faster than G-CSF-mobilized PBSC. To determine mechanisms responsible for faster hematopoietic recovery, we characterized immunophenotype and function of the GRO-mobilized grafts. PBSC mobilized by GRO4 alone or with G-CSF contained significantly more Sca-1+-c-kit+-lineage- (SKL) cells and more primitive CD34neg-SKL cells compared to cells mobilized by G-CSF and demonstrated superior competitive long-term repopulation activity, which continued to increase in secondary and tertiary recipients. GRO4-mobilized SKL cells adhered better to VCAM-1pos endothelial cells compared to G-CSF-mobilized cells. GRO4-mobilized PBSC did not migrate well to SDF-1 in vitro that was associated with higher CD26 expression. However, GRO4-mobilized SKL and KL cells homed more efficiently to marrow in vivo, which was not affected by selective CXCR4 and CD26 antagonists. These data suggest that GRO4-mobilized PBSC are superior in reconstituting long-term hematopoiesis, which results from differential mobilization of early stem cells with enhanced homing and long-term repopulating capacity. In addition, homing and engraftment of GRO4-mobilized cells is less dependent on the SDF-1/CXCR4 axis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Loos, G. Opdenakker, J. Van Damme, and P. Proost Citrullination of CXCL8 increases this chemokine's ability to mobilize neutrophils into the blood circulation Haematologica, October 1, 2009; 94(10): 1346 - 1353. [Abstract] [Full Text] [PDF] H. Kulkarni, V. C. Marconi, W. He, M. L. Landrum, J. F. Okulicz, J. Delmar, D. Kazandjian, J. Castiblanco, S. S. Ahuja, E. J. Wright, et al. 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