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Blood, 15 April 2007, Vol. 109, No. 8, pp. 3369-3376. Prepublished online as a Blood First Edition Paper on December 7, 2006; DOI 10.1182/blood-2006-06-031484. This Article Full Text (PDF) All Versions of this Article: blood-2006-06-031484v1 109/8/3369    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kvale, E. O Articles by Olweus, J. Search for Related Content PubMed PubMed Citation Articles by Kvale, E. O Articles by Olweus, J. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 26, 2006 Accepted November 30, 2006 Plasmacytoid DCs regulate recall responses by rapid induction of IL-10 in memory T cells Espen O Kvale*, Yngvar Floisand, Fridtjof Lund-Johansen, Halvor Rollag, Lorant Farkas, Smita Ghanekar, Per Brandtzaeg, Frode L Jahnsen, and Johanna Olweus Laboratory for Immunohistochemistry and Immunopathology, Institute of Pathology, University of Oslo, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway Institute of Immunology, University of Oslo, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway Section of Hematology, Department of Medicine, University of Oslo, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway Institute of Medical Microbiology, University of Oslo, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway BD Biosciences, San Jose, CA, United States * Corresponding author; email: e.o.kvale{at}medisin.uio.no. Dendritic cells (DCs) are believed to regulate T-cell mediated immunity primarily by directing differentiation of naive T cells. Here, we show that a large fraction of CD4+ memory cells produce IL-10 within the first hours after interaction with plasmacytoid DCs (PDCs). In contrast, CD11c+ DCs induce IFN- and little IL-10. IL-10-secreting T cells isolated after 36 h of culture with PDCs, suppressed antigen-induced T cell proliferation by an IL-10-dependent mechanism, but were distinct from natural and type 1 regulatory T cells. They proliferated strongly and continued to secrete IL-10 during expansion with PDCs, and after re-stimulation with immature monocyte-derived DCs or CD11c+ DCs. The IL-10-producing T cells acquired the ability to secrete high levels of IFN- after isolation and subsequent co-culture with PDCs or CD11c+ DCs. Compared to CD11c+ DCs, PDCs were superior in their ability to selectively expand T cells that produced cytokines upon repeated antigenic challenge. The DC-dependent differences in cytokine profiles were observed with viral recall antigen or staphylococcal enterotoxin B and were independent of extracellular type I interferon or IL-10. Our results show that DCs can regulate memory responses, and that PDCs rapidly induce regulatory cytokines in effector T cells that can suppress bystander activity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Sadaka, M.-A. Marloie-Provost, V. Soumelis, and P. Benaroch Developmental regulation of MHC II expression and transport in human plasmacytoid-derived dendritic cells Blood, March 5, 2009; 113(10): 2127 - 2135. [Abstract] [Full Text] [PDF] I Heier, K Malmstrom, A S Pelkonen, L P Malmberg, M Kajosaari, M Turpeinen, H Lindahl, P Brandtzaeg, F L Jahnsen, and M J Makela Bronchial response pattern of antigen presenting cells and regulatory T cells in children less than 2 years of age Thorax, August 1, 2008; 63(8): 703 - 709. [Abstract] [Full Text] [PDF]

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