SEARCH:   Advanced

Blood, 15 February 2007, Vol. 109, No. 4, pp. 1460-1471. Prepublished online as a Blood First Edition Paper on October 12, 2006; DOI 10.1182/blood-2006-07-030726. This Article Full Text (PDF) Supplemental Tables and Figure All Versions of this Article: blood-2006-07-030726v1 109/4/1460    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ghinassi, B. Articles by Migliaccio, A. R. Search for Related Content PubMed PubMed Citation Articles by Ghinassi, B. Articles by Migliaccio, A. R. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 13, 2006 Accepted September 19, 2006 The hypomorphic Gata1low mutation alters the proliferation/differentiation potential of the common megakaryocytic-erythroid progenitor Barbara Ghinassi, Massimo Sanchez, Fabrizio Martelli, Giovanni Amabile, Alessandro Maria Vannucchi, Giovanni Migliaccio, Stuart H Orkin, and Anna Rita Migliaccio* Department of Biomorphology, University G. D'Annunzio, Chieti, Italy Department of Cell Biology and Neurosciences, Istituto Superiore di Sanita, Rome, Italy Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy Department of Hematology and Oncology, University of Florence, Florence, Italy Department of Pediatric Oncology, Children's Hospital, Harvard Medical School and Howard Hughes Medical Institute, Boston, MA Department of Pathology, University of Illinois at Chicago, Chicago, IL * Corresponding author; email: migliar{at}iss.it. Recent evidence suggests that mutations in the Gata1 gene may alter the proliferation/differentiation potential of the hemopoietic progenitors. By single cell cloning and sequential replating experiments of prospectively isolated progenitor cells, we demonstrate here that the hypomorphic Gata1low mutation increases the proliferation potential of a unique class of progenitor cells, similar in phenotype to adult common erythroid/megakaryocytic progenitors (MEP), but with the "unique" capacity to generate erythroblasts, megakaryocytes and mast cells in vitro. Conversely, progenitor cells phenotypically similar to mast cell progenitors (MCP) are not detectable in the marrow from these mutants. At the single cell level, ~11% of Gata1low progenitor cells, including MEP, generate cells that will continue to proliferate in cultures for up to 4 months. In agreement with these results, tri-lineage (erythroid, megakaryocytic and mastocytic) cell lines are consistently isolated from bone marrow and spleen cells of Gata1low mice. These results confirm the crucial role played by Gata1 in hematopoietic commitment and identify, as a new target for the Gata1 action, the restriction point at which CMP became either MEP or MCP. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: On the origins of megakaryocytes John D. Crispino Blood 2007 109: 1340-1341. [Full Text] [PDF] This article has been cited by other articles: A. R. Migliaccio, F. Martelli, M. Verrucci, M. Sanchez, M. Valeri, G. Migliaccio, A. M. Vannucchi, M. Zingariello, A. Di Baldassarre, B. Ghinassi, et al. Gata1 expression driven by the alternative HS2 enhancer in the spleen rescues the hematopoietic failure induced by the hypomorphic Gata1low mutation Blood, September 3, 2009; 114(10): 2107 - 2120. [Abstract] [Full Text] [PDF] K. E. Elagib, I. S. Mihaylov, L. L. Delehanty, G. C. Bullock, K. D. Ouma, J. F. Caronia, S. L. Gonias, and A. N. Goldfarb Cross-talk of GATA-1 and P-TEFb in megakaryocyte differentiation Blood, December 15, 2008; 112(13): 4884 - 4894. [Abstract] [Full Text] [PDF] S. T. Chou, J. B. Opalinska, Y. Yao, M. A. Fernandes, A. Kalota, J. S. J. Brooks, J. K. Choi, A. M. Gewirtz, G.-a. Danet-Desnoyers, R. L. Nemiroff, et al. Trisomy 21 enhances human fetal erythro-megakaryocytic development Blood, December 1, 2008; 112(12): 4503 - 4506. [Abstract] [Full Text] [PDF] A. B. Cantor, H. Iwasaki, Y. Arinobu, T. B. Moran, H. Shigematsu, M. R. Sullivan, K. Akashi, and S. H. Orkin Antagonism of FOG-1 and GATA factors in fate choice for the mast cell lineage J. Exp. Med., March 17, 2008; 205(3): 611 - 624. [Abstract] [Full Text] [PDF] D. Metcalf, I. Majewski, S. Mifsud, L. Di Rago, and W. S. Alexander Clonogenic mast cell progenitors and their excess numbers in chimeric BALB/c mice with inactivated GATA-1 PNAS, November 20, 2007; 104(47): 18642 - 18647. [Abstract] [Full Text] [PDF] J. M. Salmon, N. J. Slater, M. A. Hall, M. P. McCormack, S. L. Nutt, S. M. Jane, and D. J. Curtis Aberrant mast-cell differentiation in mice lacking the stem-cell leukemia gene Blood, November 15, 2007; 110(10): 3573 - 3581. [Abstract] [Full Text] [PDF] M. Kobayashi and M. Yamamoto Regulation of GATA1 Gene Expression J. Biochem., July 1, 2007; 142(1): 1 - 10. [Abstract] [Full Text] [PDF] C. Slape, H. Hartung, Y.-W. Lin, J. Bies, L. Wolff, and P. D. Aplan Retroviral Insertional Mutagenesis Identifies Genes that Collaborate with NUP98-HOXD13 during Leukemic Transformation Cancer Res., June 1, 2007; 67(11): 5148 - 5155. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020