|
|
Blood, 1 April 2007, Vol. 109, No. 7, pp. 2953-2960.
Prepublished online as a Blood First Edition Paper on November 28, 2006; DOI 10.1182/blood-2006-07-033258.
 Previous Article | Next Article 
Submitted July 5, 2006
Accepted November 13, 2006
Immunostimulatory RNA oligonucleotides trigger an antigen-specific cytotoxic T cell and IgG2a response
Carole Bourquin*, Laura Schmidt, Veit Hornung, Cornelia Wurzenberger, David Anz, Nadja Sandholzer, Susanne Schreiber, Andreas Voelkl, Gunther Hartmann, and Stefan Endres
Division of Clinical Pharmacology, Dept of Internal Medicine, Ludwig-Maximilian University of Munich, Munich, Germany
Division of Clinical Pharmacology, University Hospital, University of Bonn, Bonn, Germany
* Corresponding author; email: carole.bourquin{at}med.uni-muenchen.de.
Single-stranded RNA oligonucleotides containing an immunostimulatory motif (immunostimulatory RNA, isRNA) are potent inducers of interferon- via the Toll-like receptor 7. We investigated the effect of isRNA on the development of an immune response. We show that isRNA activates dendritic cells and induces production of Th1-type cytokines both in vitro and in vivo. Cytokine production led to bystander activation of T and B cells. We further demonstrate that isRNA triggers the generation of antigen-specific cytotoxic T cells and of an IgG2a-biased antibody response to antigen in a sequence-dependent manner. In summary, we provide evidence for the first time that isRNA oligonucleotides can simultaneously activate the innate and adaptive arms of the immune system.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Bourquin, L. Schmidt, A.-L. Lanz, B. Storch, C. Wurzenberger, D. Anz, N. Sandholzer, R. Mocikat, M. Berger, H. Poeck, et al.
Immunostimulatory RNA Oligonucleotides Induce an Effective Antitumoral NK Cell Response through the TLR7
J. Immunol.,
November 15, 2009;
183(10):
6078 - 6086.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Anz, R. Thaler, N. Stephan, Z. Waibler, M. J. Trauscheid, C. Scholz, U. Kalinke, W. Barchet, S. Endres, and C. Bourquin
Activation of Melanoma Differentiation-Associated Gene 5 Causes Rapid Involution of the Thymus
J. Immunol.,
May 15, 2009;
182(10):
6044 - 6050.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. M. Grauer, J. W. Molling, E. Bennink, L. W. J. Toonen, R. P. M. Sutmuller, S. Nierkens, and G. J. Adema
TLR Ligands in the Local Treatment of Established Intracerebral Murine Gliomas
J. Immunol.,
November 15, 2008;
181(10):
6720 - 6729.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Bourquin, D. Anz, K. Zwiorek, A.-L. Lanz, S. Fuchs, S. Weigel, C. Wurzenberger, P. von der Borch, M. Golic, S. Moder, et al.
Targeting CpG Oligonucleotides to the Lymph Node by Nanoparticles Elicits Efficient Antitumoral Immunity
J. Immunol.,
September 1, 2008;
181(5):
2990 - 2998.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. J. M. Smits, P. Ponsaerts, Z. N. Berneman, and V. F. I. Van Tendeloo
The Use of TLR7 and TLR8 Ligands for the Enhancement of Cancer Immunotherapy
Oncologist,
August 1, 2008;
13(8):
859 - 875.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Lan, E. R. Kandimalla, D. Yu, L. Bhagat, Y. Li, D. Wang, F. Zhu, J. X. Tang, M. R. Putta, Y. Cong, et al.
Stabilized immune modulatory RNA compounds as agonists of Toll-like receptors 7 and 8
PNAS,
August 21, 2007;
104(34):
13750 - 13755.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Meier, G. Alter, N. Frahm, H. Sidhu, B. Li, A. Bagchi, N. Teigen, H. Streeck, H.-J. Stellbrink, J. Hellman, et al.
MyD88-Dependent Immune Activation Mediated by Human Immunodeficiency Virus Type 1-Encoded Toll-Like Receptor Ligands
J. Virol.,
August 1, 2007;
81(15):
8180 - 8191.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
