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Blood, 15 April 2007, Vol. 109, No. 8, pp. 3544-3551. Prepublished online as a Blood First Edition Paper on December 7, 2006; DOI 10.1182/blood-2006-07-035139. This Article Full Text (PDF) All Versions of this Article: blood-2006-07-035139v1 109/8/3544    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by El Nemer, W. Articles by Le Van Kim, C. Search for Related Content PubMed PubMed Citation Articles by El Nemer, W. Articles by Le Van Kim, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 12, 2006 Accepted November 30, 2006 Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell 41 integrin: role in adhesion of sickle red blood cells to endothelial cells Wassim El Nemer, Marie-Paule Wautier, Cecile Rahuel, Pierre Gane, Patricia Hermand, Frederic Galacteros, Jean-Luc Wautier, Jean-Pierre Cartron, Yves Colin, and Caroline Le Van Kim* INSERM, Paris, France Institute National de la Transfusion Sanguine, Paris, France University Paris, Paris, France Hopital Henri Mondor, APHP, Creteil, France * Corresponding author; email: levankim{at}idf.inserm.fr. Lutheran (Lu) blood group and Basal Cell Adhesion Molecule (BCAM) antigens are both carried by two glycoprotein isoforms of the immunoglobulin superfamily representing receptors for laminin 5 chain. In addition to red blood cells, Lu/BCAM are highly expressed in endothelial cells. Abnormal adhesion of red blood cells to endothelium could potentially contribute to the vaso-occlusive episodes in sickle cell disease. Considering the presence of integrin consensus binding sites in Lu/BCAM proteins, we investigated their potential interaction with integrin 41, the unique integrin expressed on immature circulating sickle red cells. Using cell adhesion assays under static and flow conditions, we demonstrated that integrin 41 expressed on transfected cells bound to chimeric Lu-Fc protein. We showed that epinephrine-stimulated sickle but not control red cells adhered to Lu-Fc via integrin 41 under flow conditions. Antibody mediated activation of integrin 41 induced adhesion of sickle red cells to primary human umbilical vein endothelial cells and this adhesion was inhibited by soluble Lu-Fc and VCAM-1-Fc proteins. This novel interaction between integrin 41 in sickle red cells, and endothelial Lu/BCAM proteins could participate in sickle cell adhesion to endothelium and potentially play a role in vaso-occlusive episodes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M.-H. Odievre, V. Bony, M. Benkerrou, C. Lapoumeroulie, C. Alberti, R. Ducrocq, E. Jacqz-Aigrain, J. Elion, and J.-P. Cartron Modulation of erythroid adhesion receptor expression by hydroxyurea in children with sickle cell disease Haematologica, April 1, 2008; 93(4): 502 - 510. [Abstract] [Full Text] [PDF] W. R. Smith, L. T. Penberthy, V. E. Bovbjerg, D. K. McClish, J. D. Roberts, B. Dahman, I. P. Aisiku, J. L. Levenson, and S. D. Roseff Daily Assessment of Pain in Adults with Sickle Cell Disease Ann Intern Med, January 15, 2008; 148(2): 94 - 101. [Abstract] [Full Text] [PDF]

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