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Blood, 15 May 2007, Vol. 109, No. 10, pp. 4511-4517.
Prepublished online as a Blood First Edition Paper on January 30, 2007; DOI 10.1182/blood-2006-07-036186.
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Submitted July 21, 2006
Accepted October 30, 2006
Physiological systemic iron metabolism in mice
deficient for duodenal Hfe
Maja Vujic Spasic, Judit Kiss, Thomas Herrmann, Regina Kessler, Jens Stolte, Bruno Galy, Birgit Rathkolb, Eckhard Wolf, Wolfgang Stremmel, Matthias W Hentze, and Martina U Muckenthaler*
Molecular Medicine Parnership Unit
Dept of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Heidelberg, Germany
Dept of Internal Medicine IV, University of Heidelberg, Heidelberg, Germany
European Molecular Biology Laboratory, Heidelberg, Germany
German Mouse Clinic, & Institute of Molecular Animal Breeding & Biotechnology, Ludwig-Maximilian-University, Germany
* Corresponding author; email: martina.muckenthaler{at}med.uni-heidelberg.de.
Mutations in the Hfe gene result in hereditary hemochromatosis (HH), a disorder characterized by increased duodenal iron absorption and tissue iron overload. Identification of a direct interaction between Hfe and transferrin receptor 1 in duodenal cells led to the hypothesis that the lack of functional Hfe in the duodenum affects TfR1-mediated serosal uptake of iron and misprogramming of the iron absorptive cells. Contrasting this view, Hfe deficiency causes inappropriately low expression of the hepatic iron hormone hepcidin, which causes increased duodenal iron absorption. We specifically ablated Hfe expression in mouse enterocytes using Cre/LoxP technology. Mice with efficient deletion of Hfe in crypt- and villi-enterocytes maintain physiological iron metabolism with wild type unsaturated iron binding capacity, hepatic iron levels and hepcidin mRNA expression. Furthermore, the expression of genes encoding the major intestinal iron transporters is unchanged in duodenal Hfe-deficient mice. Our data demonstrate that intestinal Hfe is dispensable for the physiological control of systemic iron homeostasis under steady state conditions. These findings exclude a primary role for duodenal Hfe in the pathogenesis of HH, and support the model according to which Hfe is required for appropriate expression of the "iron hormone" hepcidin which then controls intestinal iron absorption.
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