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Blood, 1 August 2007, Vol. 110, No. 3, pp. 877-885. Prepublished online as a Blood First Edition Paper on April 11, 2007; DOI 10.1182/blood-2006-07-036202. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: blood-2006-07-036202v1 110/3/877    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Grote, K. Articles by Schieffer, B. Search for Related Content PubMed PubMed Citation Articles by Grote, K. Articles by Schieffer, B. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 19, 2006 Accepted April 4, 2007 The angiogenic factor CCN1 promotes adhesion and migration of circulating CD34+ progenitor cells: potential role in angiogenesis and endothelial regeneration Karsten Grote*, Gustavo Salguero, Matthias Ballmaier, Marc Dangers, Helmut Drexler, and Bernhard Schieffer Dept of Cardiology & Angiology, Medical School Hannover, Hannover, Germany Dept of Pediatric Hematology, Medical School Hannover, Hannover, Germany Dept of Nephrology, Medical School Hannover, Hannover, Germany * Corresponding author; email: grote.karsten{at}mh-hannover.de. Tissue regeneration involves the formation of new blood vessels regulated by angiogenic factors. We recently reported that the expression of the angiogenic factor CCN1 is up-regulated under various pathophysiological conditions within the cardiovascular system. Since CD34+ progenitor cells participate in cardiovascular tissue regeneration, we investigated if CCN1--which we first time detected in human plasma--promotes the recruitment of CD34+ progenitor cells to endothelial cells thereby enhancing endothelial proliferation and neo-vascularization. Here, we demonstrate that CCN1 and supernatants from CCN1-stimulated human CD34+ progenitor cells promoted proliferation of endothelial cells and angiogenesis in vitro and in vivo. In addition, CCN1 induced migration and transendothelial migration of CD34+ cells and the release of multiple growth factors, chemokines and matrix metalloproteinase-9 (MMP-9) from these cells. Moreover, the CCN1-specific integrins M2 and V3 are expressed on CD34+ cells and CCN1 stimulated integrin-dependent signaling. Furthermore, integrin antagonists (RGD-peptides) suppressed both binding of CCN1 to CD34+ cells and CCN1-induced adhesion of CD34+ cells to endothelial cells. These data suggest that CCN1 promotes integrin-dependent recruitment of CD34+ progenitor cells to endothelial cells which may contribute to paracrine effects on angiogenesis and tissue regeneration. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. Bonuccelli, M. C. Casimiro, F. Sotgia, C. Wang, M. Liu, S. Katiyar, J. Zhou, E. Dew, F. Capozza, K. M. Daumer, et al. Caveolin-1 (P132L), a Common Breast Cancer Mutation, Confers Mammary Cell Invasiveness and Defines a Novel Stem Cell/Metastasis-Associated Gene Signature Am. J. Pathol., May 1, 2009; 174(5): 1650 - 1662. [Abstract] [Full Text] [PDF]

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