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Blood, 15 March 2007, Vol. 109, No. 6, pp. 2514-2520. Prepublished online as a Blood First Edition Paper on November 16, 2006; DOI 10.1182/blood-2006-07-036715. This Article Full Text (PDF) All Versions of this Article: blood-2006-07-036715v1 109/6/2514    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Takamatsu, H. Articles by Nakao, S. Search for Related Content PubMed PubMed Citation Articles by Takamatsu, H. Articles by Nakao, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 25, 2006 Accepted October 24, 2006 Specific antibodies to moesin, a membrane-cytoskeleton linker protein, are frequently detected in patients with acquired aplastic anemia Hiroyuki Takamatsu, Xingmin Feng, Tatsuya Chuhjo, Xuzhang Lu, Chiharu Sugimori, Katsuya Okawa, Miyuki Yamamoto, Shoichi Iseki, and Shinji Nakao* Cellular Transplantation Biology, Div. of Cancer Medicine, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan Internal Medicine, NTT WEST Kanazawa Hospital, Kanazawa, Japan Biomolecular Characterization Unit, Kyoto University Graduate School of Medicine, Kyoto, Japan Department of Histology and Embryology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan * Corresponding author; email: snakao{at}med3.m.kanazawa-u.ac.jp. To identify novel autoantibodies in acquired aplastic anemia (AA), we screened the sera of AA patients possessing small populations of paroxysmal nocturnal hemoglobinuria (PNH)-type cells for the presence of antibodies (Abs) which recognize proteins derived from a leukemia cell line, UT-7. Immunoblotting using proteins derived from lysates or culture supernatants of UT-7 cells revealed the presence of IgG Abs specific to an 80 kD protein. Peptide mass fingerprinting identified this 80 kD protein as moesin. Enzyme-linked immunosorbent assay (ELISA) using recombinant moesin showed high titers of anti-moesin Abs in 25 (37%) of 67 AA patients. Moesin was secreted from several myeloid leukemia cell lines other than UT-7, such as OUN-1 and K562, as an exosomal protein. The presence of anti-moesin Abs was significantly correlated with the presence of PNH-type cells and anti-diazepam-binding inhibitor-related protein-1 (DRS-1) Abs. AA patients that did not show any of these three markers tended to respond poorly to immunosuppressive therapy. These findings suggest that a B-cell response to moesin, possibly derived from hematopoietic cells, frequently occurs in patients with AA and that detection of anti-moesin Abs in combination with other markers may be useful in diagnosing immune pathophysiology in AA patients. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. L. Espinoza, H. Takamatsu, X. Lu, Z. Qi, and S. Nakao Anti-moesin antibodies derived from patients with aplastic anemia stimulate monocytic cells to secrete TNF-{alpha} through an ERK1/2-dependent pathway Int. Immunol., August 1, 2009; 21(8): 913 - 923. [Abstract] [Full Text] [PDF] H. Takamatsu, J. L. Espinoza, X. Lu, Z. Qi, K. Okawa, and S. Nakao Anti-Moesin Antibodies in the Serum of Patients with Aplastic Anemia Stimulate Peripheral Blood Mononuclear Cells to Secrete TNF-{alpha} and IFN-{gamma} J. Immunol., January 1, 2009; 182(1): 703 - 710. [Abstract] [Full Text] [PDF]

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