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Blood, 1 June 2007, Vol. 109, No. 11, pp. 4832-4838. Prepublished online as a Blood First Edition Paper on February 15, 2007; DOI 10.1182/blood-2006-07-037481. This Article Full Text (PDF) All Versions of this Article: blood-2006-07-037481v1 109/11/4832    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fujiwara, M. Articles by Takiguchi, M. Search for Related Content PubMed PubMed Citation Articles by Fujiwara, M. Articles by Takiguchi, M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 26, 2006 Accepted February 9, 2007 HIV-1-specific CTLs effectively suppress replication of HIV-1 in HIV-1-infected macrophages Mamoru Fujiwara and Masafumi Takiguchi* Division of Viral Immunology, Center for AIDS Research, Kumamoto University, Kumamoto, Japan * Corresponding author; email: masafumi{at}kumamoto-u.ac.jp. Both CD4+ T cells and macrophages are major reservoirs of HIV-1. Previous study showed that HIV-1-specific cytolytic T lymphocytes (CTLs) hardly recognize HIV-1-infected CD4+ T cells because of Nef-mediated HLA class I down-regulation, suggesting that HIV-1 escapes from HIV-1-specific CTLs and continues to replicate in HIV-1-infected donors. On the other hand, the CTL recognition of HIV-1-infected macrophages and the effect of Nef-mediated HLA class I down-regulation on this recognition still remain unclear. We show a strong HIV-1 antigen presentation by HIV-1-infected macrophages. HIV-1-specific CTLs had strong abilities to suppress HIV-1R5 virus replication in HIV-1-infected macrophages and to kill HIV-1R5-infected macrophages. Nef-mediated HLA class I down-regulation minimally influenced the recognition of HIV-1-infected macrophages by HIV-1-specific CTLs. In addition, HIV-1-infected macrophages had a stronger ability to stimulate the proliferation of HIV-1-specific CTLs than HIV-1-infected CD4+ T cells. Thus, the effect of Nef-mediated HLA class I down-regulation was less critical with respect to the recognition by HIV-1-specific CTLs of HIV-infected macrophages than that of HIV-1-infected CD4+ T cells. These findings support the idea that the strong HIV-1 antigen presentation by HIV-1-infected macrophages is one of the mechanisms mediating effective induction of HIV-1-specific CTLs in the acute and early chronic phases of HIV-1 infection. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. Zheng, M. Fujiwara, T. Ueno, S. Oka, and M. Takiguchi Strong Ability of Nef-Specific CD4+ Cytotoxic T Cells To Suppress Human Immunodeficiency Virus Type 1 (HIV-1) Replication in HIV-1-Infected CD4+ T Cells and Macrophages J. Virol., August 1, 2009; 83(15): 7668 - 7677. [Abstract] [Full Text] [PDF] J. B. Sacha, J. P. Giraldo-Vela, M. B. Buechler, M. A. Martins, N. J. Maness, C. Chung, L. T. Wallace, E. J. Leon, T. C. Friedrich, N. A. Wilson, et al. Gag- and Nef-specific CD4+ T cells recognize and inhibit SIV replication in infected macrophages early after infection PNAS, June 16, 2009; 106(24): 9791 - 9796. [Abstract] [Full Text] [PDF] M. Fujiwara, J. Tanuma, H. Koizumi, Y. Kawashima, K. Honda, S. Mastuoka-Aizawa, S. Dohki, S. Oka, and M. Takiguchi Different Abilities of Escape Mutant-Specific Cytotoxic T Cells To Suppress Replication of Escape Mutant and Wild-Type Human Immunodeficiency Virus Type 1 in New Hosts J. Virol., January 1, 2008; 82(1): 138 - 147. [Abstract] [Full Text] [PDF]

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