|
|
Blood, 15 April 2007, Vol. 109, No. 8, pp. 3225-3234.
Prepublished online as a Blood First Edition Paper on December 14, 2006; DOI 10.1182/blood-2006-07-037838.
 Previous Article | Next Article 
Submitted July 28, 2006
Accepted December 7, 2006
Interrelation between polyploidization and megakaryocyte differentiation: a gene profiling approach
Hana Raslova*, Audrey Kauffmann, Dalila Sekkai, Hugues Ripoche, Frederic Larbret, Thomas Robert, Diana Tronik Leroux, Guido Kroemer, Najet Debili, Philippe Dessen, Vladimir Lazar, and William Vainchenker
INSERM, Institut Gustave Roussy, Villejuif, France
CNRS, Institut Gustave Roussy, Villejuif, France
Plateforme de genomique fonctionnelle, IFR54, Institut Gustave Roussy, Villejuif, France
CEA, Evry, France
* Corresponding author; email: hraslova{at}igr.fr.
Polyploidization is a part of the normal developmental process leading to platelet production during megakaryocyte (MK) differentiation. Ploidization is mainly involved in cell enlargement but it is poorly known whether gene expression is modified during MK ploidization. In this study, human MKs were grown from CD34+ cells in the presence of TPO and sorted according to their ploidy level. A pangenomic microarray technique was applied to compare gene expression in 2N, 4N, 8N and 16N sorted MKs. Using a hierarchical clustering, we demonstrated that 2N and 4N MKs or 8N and 16N MKs are two different close populations with 105 discriminating genes. In the second approach, we determined the profile of genes that were continuously down- and up-regulated during polyploidization. Among the 100 down-regulated genes, 24 corresponded to genes involved in DNA replication and repair. The great majority of up-regulated genes corresponded to genes directly involved in platelet functions, such as genes encoding specific platelet glycoproteins and  -granule proteins, actin and microtubule cytoskeleton, factors involved in signaling and transport proteins. Altogether, these results suggest that MK polyploidization per se does not regulate gene expression but is intrinsically included in the differentiation process.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
Related Article in Blood Online:
-
MEGAprofiles provide big insightsinto platelet function
- Wadie F. Bahou
Blood 2007 109: 3129-3130.
[Full Text]
[PDF]
This article has been cited by other articles:
|
|
|
|
 
J. Swire, S. Fuchs, J. G. Bundy, and A. M. Leroi
The cellular geometry of growth drives the amino acid economy of Caenorhabditis elegans
Proc R Soc B,
August 7, 2009;
276(1668):
2747 - 2754.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E.-c. Cheng, Q. Luo, E. M. Bruscia, M. J. Renda, J. A. Troy, S. A. Massaro, D. Tuck, V. Schulz, S. M. Mane, N. Berliner, et al.
Role for MKL1 in megakaryocytic maturation
Blood,
March 19, 2009;
113(12):
2826 - 2834.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Yamane, T. Nakamura, H. Suzuki, M. Ito, Y. Ohnishi, Y. Ikeda, and Y. Miyakawa
Interferon-{alpha}2b-induced thrombocytopenia is caused by inhibition of platelet production but not proliferation and endomitosis in human megakaryocytes
Blood,
August 1, 2008;
112(3):
542 - 550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Cazzola
Molecular basis of thrombocytosis
Haematologica,
May 1, 2008;
93(5):
646 - 648.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Gilles, R. Guieze, D. Bluteau, V. Cordette-Lagarde, C. Lacout, R. Favier, F. Larbret, N. Debili, W. Vainchenker, and H. Raslova
P19INK4D links endomitotic arrest and megakaryocyte maturation and is regulated by AML-1
Blood,
April 15, 2008;
111(8):
4081 - 4091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. G. Fuhrken, C. Chen, P. A. Apostolidis, M. Wang, W. M. Miller, and E. T. Papoutsakis
Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesis
Physiol Genomics,
April 1, 2008;
33(2):
159 - 169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
