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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5371-5379. Prepublished online as a Blood First Edition Paper on March 1, 2007; DOI 10.1182/blood-2006-08-038422. This Article Full Text (PDF) All Versions of this Article: blood-2006-08-038422v1 109/12/5371    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Piccioli, D. Articles by Wack, A. Search for Related Content PubMed PubMed Citation Articles by Piccioli, D. Articles by Wack, A. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 2, 2006 Accepted February 16, 2007 Functional specialization of human circulating CD16 and CD1c myeloid dendritic cell subsets Diego Piccioli, Simona Tavarini, Erica Borgogni, Veronica Steri, Sandra Nuti, Chiara Sammicheli, Monia Bardelli, Daniela Montagna, Franco Locatelli, and Andreas Wack* Molecular Immunology Department, Research Center, Novartis Vaccines, Siena, Italy Molecular Biology Department, Universita degli Studi, Siena, Italy Department of Pediatrics, University of Pavia, Pediatric Hematology Unit, IRCCS Policlinico San Matteo, Pavia, Italy * Corresponding author; email: andreas_wack{at}chiron.com. Human blood contains two populations of dendritic cells (DCs) termed plasmacytoid and myeloid. Myeloid DCs (mDCs) are subdivided into three subsets using the surface markers CD16, CD1c, and BDCA-3. Their differential role as pathogen sentinels and adjuvant targets was tested by extensive phenotypic and functional analysis. We show that mDC subsets are immature and express mRNA for most TLRs, except for TLR3 in CD16-mDCs. The most represented subsets, CD16 and CD1c-mDCs, are similarly responsive to all TLR agonists. Among 31 cytokines tested, both subsets produce CXCL8(IL-8)/TNF-/IL-6/CCL3(MIP-1)/CCL4(MIP-1)/IL-1. CXCL8(IL-8) is the predominant cytokine produced by CD1c-mDCs upon TLR engagement, whereas all other cytokines, particularly TNF-, are secreted in 10-fold to 100-fold higher amounts by CD16-mDCs. In addition, CD16-mDCs co-cultured with HUVECs induce a significantly higher production of CXCL10(IP-10), GM-CSF and G-CSF than CD1c-mDCs. We also show that IL-3 and type-I interferons are stimuli specifically for DC maturation rather than cytokine secretion, while TNF- is almost ineffective in inducing either function, suggesting a possible mechanism of T cell-DC cross-talk and of rapid induction of APC function during viral infection rather than inflammation. In conclusion CD16-mDCs show strong pro-inflammatory activity, while CD1c-mDCs appear to be mainly inducers of chemotaxis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Piccioli, C. Sammicheli, S. Tavarini, S. Nuti, E. Frigimelica, A. G.O. Manetti, A. Nuccitelli, S. Aprea, S. Valentini, E. Borgogni, et al. Human plasmacytoid dendritic cells are unresponsive to bacterial stimulation and require a novel type of cooperation with myeloid dendritic cells for maturation Blood, April 30, 2009; 113(18): 4232 - 4239. [Abstract] [Full Text] [PDF] M. Abt, E. Gassert, and S. Schneider-Schaulies Measles virus modulates chemokine release and chemotactic responses of dendritic cells J. Gen. Virol., April 1, 2009; 90(4): 909 - 914. [Abstract] [Full Text] [PDF] C. Huysamen, J. A. Willment, K. M. Dennehy, and G. D. Brown CLEC9A Is a Novel Activation C-type Lectin-like Receptor Expressed on BDCA3+ Dendritic Cells and a Subset of Monocytes J. Biol. Chem., June 13, 2008; 283(24): 16693 - 16701. [Abstract] [Full Text] [PDF]

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