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Blood, 15 February 2007, Vol. 109, No. 4, pp. 1636-1642. Prepublished online as a Blood First Edition Paper on October 12, 2006; DOI 10.1182/blood-2006-08-039024. This Article Full Text (PDF) All Versions of this Article: blood-2006-08-039024v1 109/4/1636    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Natkunam, Y. Articles by Levy, R. Search for Related Content PubMed PubMed Citation Articles by Natkunam, Y. Articles by Levy, R. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 1, 2006 Accepted October 3, 2006 The oncoprotein LMO2 is expressed in normal germinal center B-cells and in human B-cell lymphomas Yasodha Natkunam*, Shuchun Zhao, David Y. Mason, Jun Chen, Behnaz Taidi, Margaret Jones, Anne S Hammer, Stephen Hamilton-Dutoit, Izidore S. Lossos, and Ronald Levy Dept of Pathology, Stanford University School of Medicine, Stanford, CA Nuffield Dept of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, United Kingdom Sylvester Comprehensive Cancer Center, University of Miami, Miami, FL Dept of Medicine, Division of Oncology, Stanford University School of Medicine, Stanford, CA Institute of Pathology, Aarhus University Hospital, Aarhus, Denmark * Corresponding author; email: yaso{at}stanford.edu. We previously developed a multivariate model based on the RNA expression of six genes - LMO2, BCL6, FN1, CCND2, SCYA3 and BCL2 - that predicts survival in DLBCL patients. Since LMO2 emerged as the strongest predictor of superior outcome, we generated a monoclonal anti-LMO2 antibody in order to study its tissue expression pattern. Immunohistological analysis of over 1200 normal and neoplastic tissue and cell-lines showed that LMO2 protein is expressed as a nuclear marker in normal germinal center (GC) B-cells, GC-derived B-cell lines and in a subset of GC-derived B-cell lymphomas. LMO2 was also expressed in erythroid and myeloid precursors and in megakaryocytes, and also in lymphoblastic and acute myeloid leukemias. It was rarely expressed in mature T, NK and plasma cell neoplasms and was absent from non-hematolymphoid tissues except for endothelial cells. Hierarchical cluster analysis of immunohistologic data in DLBCL demonstrated that the expression profile of the LMO2 protein was similar to that of other GC-associated proteins (HGAL, BCL6 and CD10) but different from that of non-GC proteins (MUM1/IRF4 and BCL2). Our results warrant inclusion of LMO2 in multivariate analyses to construct a clinically applicable immunohistologic algorithm for predicting survival in patients with DLBCL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: W. W.L. Choi, D. D. Weisenburger, T. C. Greiner, M. A. Piris, A. H. Banham, J. Delabie, R. M. Braziel, H. Geng, J. Iqbal, G. Lenz, et al. A New Immunostain Algorithm Classifies Diffuse Large B-Cell Lymphoma into Molecular Subtypes with High Accuracy Clin. Cancer Res., September 1, 2009; 15(17): 5494 - 5502. [Abstract] [Full Text] [PDF] M. E. Salama, I. S. Lossos, R. A. Warnke, and Y. Natkunam Immunoarchitectural Patterns in Nodal Marginal Zone B-Cell Lymphoma: A Study of 51 Cases Am J Clin Pathol, July 1, 2009; 132(1): 39 - 49. [Abstract] [Full Text] [PDF] A. Appert, C.-H. Nam, N. Lobato, E. Priego, R. N. Miguel, T. Blundell, L. Drynan, H. Sewell, T. Tanaka, and T. Rabbitts Targeting LMO2 with a Peptide Aptamer Establishes a Necessary Function in Overt T-Cell Neoplasia Cancer Res., June 1, 2009; 69(11): 4784 - 4790. [Abstract] [Full Text] [PDF] J. Zhang, D. D. Jima, C. Jacobs, R. Fischer, E. Gottwein, G. Huang, P. L. Lugar, A. S. Lagoo, D. A. Rizzieri, D. R. Friedman, et al. Patterns of microRNA expression characterize stages of human B-cell differentiation Blood, May 7, 2009; 113(19): 4586 - 4594. [Abstract] [Full Text] [PDF] R. Malumbres, K. A. Sarosiek, E. Cubedo, J. W. Ruiz, X. Jiang, R. D. Gascoyne, R. Tibshirani, and I. S. Lossos Differentiation stage-specific expression of microRNAs in B lymphocytes and diffuse large B-cell lymphomas Blood, April 16, 2009; 113(16): 3754 - 3764. [Abstract] [Full Text] [PDF] D. Gratzinger, S. Zhao, R. West, R. V. Rouse, H. Vogel, E. C. Gil, R. Levy, I. S. Lossos, and Y. Natkunam The Transcription Factor LMO2 Is a Robust Marker of Vascular Endothelium and Vascular Neoplasms and Selected Other Entities Am J Clin Pathol, February 1, 2009; 131(2): 264 - 278. [Abstract] [Full Text] [PDF] D. Gaucher, R. Therrien, N. Kettaf, B. R. Angermann, G. Boucher, A. Filali-Mouhim, J. M. Moser, R. S. Mehta, D. R. Drake III, E. Castro, et al. Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses J. Exp. Med., December 22, 2008; 205(13): 3119 - 3131. [Abstract] [Full Text] [PDF] Y. Natkunam, P. Farinha, E. D. Hsi, C. P. Hans, R. Tibshirani, L. H. Sehn, J. M. Connors, D. Gratzinger, M. Rosado, S. Zhao, et al. LMO2 Protein Expression Predicts Survival in Patients With Diffuse Large B-Cell Lymphoma Treated With Anthracycline-Based Chemotherapy With and Without Rituximab J. Clin. Oncol., January 20, 2008; 26(3): 447 - 454. [Abstract] [Full Text] [PDF] R. J. Marinelli, K. Montgomery, C. L. Liu, N. H. Shah, W. Prapong, M. Nitzberg, Z. K. Zachariah, G. J. Sherlock, Y. Natkunam, R. B. West, et al. The Stanford Tissue Microarray Database Nucleic Acids Res., January 11, 2008; 36(suppl_1): D871 - D877. [Abstract] [Full Text] [PDF] Y. Natkunam The Biology of the Germinal Center Hematology, January 1, 2007; 2007(1): 210 - 215. [Abstract] [Full Text] [PDF]

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