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Blood, 15 June 2007, Vol. 109, No. 12, pp. 5327-5336. Prepublished online as a Blood First Edition Paper on March 7, 2007; DOI 10.1182/blood-2006-08-043109. This Article Full Text (PDF) Supplemental Table All Versions of this Article: blood-2006-08-043109v1 109/12/5327    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bixel, M. G. Articles by Vestweber, D. Search for Related Content PubMed PubMed Citation Articles by Bixel, M. G. Articles by Vestweber, D. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 21, 2006 Accepted February 26, 2007 A CD99-related antigen on endothelial cells mediates neutrophil, but not lymphocyte extravasation in vivo M. Gabriele Bixel, Bjorn Petri, Alexander G Khandoga, Andrej Khandoga, Karen Wolburg-Buchholz, Hartwig Wolburg, Sigrid Marz, Fritz Krombach, and Dietmar Vestweber* Institute of Cell Biology, ZMBE, IZKF Munster, University of Munster, Munster, Germany Max-Planck-Institute of Molecular Biomedicine, Munster, Germany Institute for Surgical Research, University of Munich, Munich, Germany Institute of Pathology, University of Tubingen, Tubingen, Germany * Corresponding author; email: vestweb{at}mpi-muenster.mpg.de. CD99 is a long known leukocyte antigen that does not belong to any of the known protein families. It was recently found on endothelial cells where it mediates transendothelial migration of human monocytes and lymphocyte recruitment into inflamed skin in the mouse. Here we show that a recently cloned, widely expressed antigen of unknown function with moderate sequence homology to CD99, called CD99L2, is expressed on mouse leukocytes and endothelial cells. Using antibodies, we found that CD99L2 and CD99 are involved in transendothelial migration of neutrophils in vitro and in the recruitment of neutrophils into inflamed peritoneum. Intravital and electron microscopy of cremaster venules revealed that blocking CD99L2 inhibited leukocyte transmigration through the vessel wall (diapedesis) at the level of the perivascular basement membrane. Surprisingly and in contrast to CD99, CD99L2 was not relevant for the extravasation of lymphocytes into inflamed tissue. Although each protein promoted cell aggregation of transfected cells, endothelial CD99 and CD99L2 participated in neutrophil extravasation independent of these proteins on neutrophils. Our results establish CD99L2 as a new endothelial surface protein involved in neutrophil extravasation. In addition, this is the first evidence for a role of CD99 and CD99L2 in the process of leukocyte-diapedesis in vivo. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: To transmigrate or not to transmigrate Klaus Ley Blood 2007 109: 5072-5073. [Full Text] [PDF] This article has been cited by other articles: J. Carvalho, R. Pereira, and Y. Shoenfeld Vaccination, atherosclerosis and systemic lupus erythematosus Lupus, November 1, 2009; 18(13): 1209 - 1212. [Abstract] [PDF] W. A. Muller Mechanisms of Transendothelial Migration of Leukocytes Circ. Res., July 31, 2009; 105(3): 223 - 230. [Abstract] [Full Text] [PDF] N. Bardin, M. 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Vestweber, T. J.C. van Berkel, and J. Kuiper Vaccination against CD99 inhibits atherogenesis in low-density lipoprotein receptor-deficient mice Cardiovasc Res, June 1, 2008; 78(3): 590 - 596. [Abstract] [Full Text] [PDF] Z. Mamdouh, G. E. Kreitzer, and W. A. Muller Leukocyte transmigration requires kinesin-mediated microtubule-dependent membrane trafficking from the lateral border recycling compartment J. Exp. Med., April 14, 2008; 205(4): 951 - 966. [Abstract] [Full Text] [PDF] A. Woodfin, M.-B. Voisin, and S. Nourshargh PECAM-1: A Multi-Functional Molecule in Inflammation and Vascular Biology Arterioscler Thromb Vasc Biol, December 1, 2007; 27(12): 2514 - 2523. [Abstract] [Full Text] [PDF] R. M. Rao, L. Yang, G. Garcia-Cardena, and F. W. Luscinskas Endothelial-Dependent Mechanisms of Leukocyte Recruitment to the Vascular Wall Circ. Res., August 3, 2007; 101(3): 234 - 247. [Abstract] [Full Text] [PDF]

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