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Blood, 15 September 2007, Vol. 110, No. 6, pp. 1960-1969. Prepublished online as a Blood First Edition Paper on May 17, 2007; DOI 10.1182/blood-2006-09-045807. This Article Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2006-09-045807v1 110/6/1960    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cassese, G. Articles by Di Rosa, F. Search for Related Content PubMed PubMed Citation Articles by Cassese, G. Articles by Di Rosa, F. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 5, 2006 Accepted May 11, 2007 Bone marrow CD8 cells down-modulate membrane IL-7R expression and exhibit increased STAT-5 and p38 MAPK phosphorylation in the organ environment Giuliana Cassese, Elisabetta Parretta, Laura Pisapia, Angela Santoni, John Guardiola, and Francesca Di Rosa* Institute of Genetics and Biophysics, "Adriano Buzzati-Traverso", Consiglio Nazionale delle Ricerche, Naples, Italy Department of Experimental Medicine, Istituto Pasteur-Fondazione Cenci Bolognetti, University of Rome "La Sapienza", Rome, Italy Institute of Molecular Biology and Pathology, Consiglio Nazionale delle Ricerche, Rome, Italy * Corresponding author; email: dirosa{at}igb.cnr.it. By comparing mature CD8 cell turn-over in different organs, we previously demonstrated that CD8 cells proliferate predominantly in the bone marrow (BM). To investigate the mechanisms underlying such increased turn-over, we compared here BM, lymph nodes (LN) and spleen CD8 cells from untreated C57BL/6 mice as regards in vivo proliferation within the organ; in vitro response to IL-7, IL-15, IL-21; ex vivo expression of membrane CD127 (IL-7R), intra-cellular Bcl-2, phospho-STAT-5, phospho-p38 MAPK; in vivo proliferation upon adoptive transfer. Into the BM, the proliferation rate was increased for either total CD8 cells or individual CD44 and CD122 subsets. In contrast, purified CD8+ cells from the BM did not show an enhanced in vitro proliferative response to IL-7, IL-15, IL-21 as compared to corresponding spleen cells. After transfer and polyI:C treatment, both spleen- and BM-derived CD8 cells from congenic donors proliferated about 2-times more in the recipient BM than in spleen and LN. Our results suggest that BM CD8 cells are not committed to self-renewal, but rather are stimulated in the organ. Molecular events constantly induced in the CD8 cells within the BM of untreated mice include increase of both phosphorylated STAT-5 and phosphorylated p38 intracellular levels, reduction of CD127 membrane expression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Parretta, G. Cassese, A. Santoni, J. Guardiola, A. Vecchio, and F. Di Rosa Kinetics of In Vivo Proliferation and Death of Memory and Naive CD8 T Cells: Parameter Estimation Based on 5-Bromo-2'-Deoxyuridine Incorporation in Spleen, Lymph Nodes, and Bone Marrow J. Immunol., June 1, 2008; 180(11): 7230 - 7239. [Abstract] [Full Text] [PDF]

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