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Blood, 1 May 2007, Vol. 109, No. 9, pp. 3922-3928. Prepublished online as a Blood First Edition Paper on January 25, 2007January 30, 2007; DOI 10.1182/blood-2006-09-046391. This Article Full Text (PDF) Supplemental Table All Versions of this Article: blood-2006-09-046391v1 blood-2006-09-046391v2 109/9/3922    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sakhinia, E. Articles by Byers, R. J Search for Related Content PubMed PubMed Citation Articles by Sakhinia, E. Articles by Byers, R. J Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 18, 2006 Accepted December 22, 2006 Clinical quantitation of diagnostic and predictive gene expression levels in follicular and diffuse large B-cell lymphoma by RT-PCR gene expression profiling Ebrahim Sakhinia, Caroline Glennie, Judith A Hoyland, Lia P Menasce, Gerard Brady, Crispin Miller, John A Radford, and Richard J Byers* Division of Regenerative Medicine, School of Medicine, Faculty of Medical & Human Sciences, University of Manchester, Manchester, United Kingdom Department of Histopathology, Christie Hospital NHS Trust, Manchester, United Kingdom Epistem Ltd., Incubator Building, Manchester, United Kingdom Paterson Institute for Cancer Research, Manchester, United Kingdom Cancer Research UK Department of Medical Oncology, University of Manchester & Christie Hospital NHS Trust, Manchester, United Kingdom * Corresponding author; email: r.byers{at}manchester.ac.uk. Recent microarray gene expression profiling studies have identified gene signatures predictive of outcome, so-called "Indicator" genes, for diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL). However, measurement of these genes in routine practice remains difficult. We applied real-time PCR, to polyA cDNAs prepared from 106 archived human frozen lymph nodes (63 of FL, 25 of DLBCL, 10 reactive lymph nodes and 4 cases with paired samples of FL (4) and subsequent DLBCL (4)). Reverse transcription and polyA RT-PCR was performed and resultant cDNA probed by real-time PCR for 36 candidate Indicator genes, selected from microarray studies. Nine genes showed statistically significant different expression between FL and DLBCL, including Cyclin B, COL3A1, NPM3, H731, PKC.B1, OVGL, ZFPC150, HLA-DQ-a, and XPB. Of these, Cyclin B, NPM3, and COL3A1 were higher in DLBCL. Six genes showed statistically significant higher expression in the neoplastic nodes compared to reactive nodes, namely PKCB-1, BCL-6, EAR2, ZFX, Cyclin B, YY.1. High levels of YY.1 were associated with a shorter survival interval in both FL and DLBCL. The method is simple, sensitive and robust, facilitating routine use and may be used as a platform for clinical measurement of prognostic gene signatures. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Gene expression levels in lymphoma Philip S. Rosenberg Blood 2007 109: 3621. [Full Text] [PDF] This article has been cited by other articles: R. J. Byers, E. Sakhinia, P. Joseph, C. Glennie, J. A. Hoyland, L. P. Menasce, J. A. Radford, and T. Illidge Clinical quantitation of immune signature in follicular lymphoma by RT-PCR-based gene expression profiling Blood, May 1, 2008; 111(9): 4764 - 4770. [Abstract] [Full Text] [PDF]

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