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Blood, 15 April 2007, Vol. 109, No. 8, pp. 3342-3350. Prepublished online as a Blood First Edition Paper on December 19, 2006; DOI 10.1182/blood-2006-09-046698. This Article Full Text (PDF) Supplemental Table and Figures All Versions of this Article: blood-2006-09-046698v1 109/8/3342    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Thomas, M. Articles by Allman, D. Search for Related Content PubMed PubMed Citation Articles by Thomas, M. Articles by Allman, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 11, 2006 Accepted December 2, 2006 Notch activity synergizes with B cell receptor and CD40 signaling to enhance B cell activation Matthew Thomas, Marco Calamito, Bhaskar Srivastava, Ivan Maillard, Warren S Pear, and David Allman* Dept of Pathology & Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA Institute for Medicine & Engineering, University of Pennsylvania School of Medicine, Philadelphia, PA * Corresponding author; email: dallman{at}mail.med.upenn.edu. How diverse environmental cues are integrated to regulate B cell activation and development remains poorly understood. Here we show that Notch activity synergizes with B cell receptor and/or CD40 signaling to enhance several aspects of B cell activation and function. We find that co-stimulation of follicular B cells with the Notch ligand Delta-like-1 leads to significant increases in BCR- and CD40-mediated proliferation, and enhances production of IgG1+ cells in vitro and in vivo. We further find that co-engagement of Notch and the BCR results in increased activation of the MAPK pathway, and MAPK and Notch inhibitors prevent B cell activation events mediated by co-engagement of Notch and the BCR. These data suggest that the BCR and CD40 signaling pathways collaborate with the Notch pathway to optimize B cell activation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. A. Santos, L. M. Sarmento, M. Rebelo, A. A. Doce, I. Maillard, A. Dumortier, H. Neves, F. Radtke, W. S. Pear, L. Parreira, et al. Notch1 engagement by Delta-like-1 promotes differentiation of B lymphocytes to antibody-secreting cells PNAS, September 25, 2007; 104(39): 15454 - 15459. [Abstract] [Full Text] [PDF]

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