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Blood, 1 June 2007, Vol. 109, No. 11, pp. 4806-4809.
Prepublished online as a Blood First Edition Paper on February 20, 2007; DOI 10.1182/blood-2006-09-047449.
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Submitted September 15, 2006
Accepted February 9, 2007
The novel inhibitory receptor G6B is expressed on the surface of platelets and attenuates platelet function in vitro
Stephen A Newland, Iain C Macaulay, R Andres Floto, Edwin C de Vet, Willem H Ouwehand, Nicholas A Watkins, Paul A Lyons, and R Duncan Campbell*
Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
National Blood Service Cambridge & Department of Haematology, University of Cambridge, Cambridge, United Kingdom
MRC Rosalind Franklin Centre for Genomics Research, Hinxton, United Kingdom
Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom
* Corresponding author; email: duncan.campbell{at}dpag.ox.ac.uk.
The G6B cell surface receptor, which contains a single Ig-like domain, has been shown to bind to SHP1 and SHP2 after phosphorylation of two ITIM motifs in its cytoplasmic tail, classifying this protein as a new member of the family of inhibitory receptors. In this study we demonstrate by Real-Time PCR and Western blot analysis that G6B is expressed on platelets. Cross-linking of G6B with polyclonal antisera has a significant inhibitory effect on platelet aggregation and activation by agonists such as ADP and Collagen Related Peptide (CRP). This inhibitory function of G6B appears to operate in a calcium independent manner. Our results suggest that G6B represents a novel inhibitory receptor found on the surface of platelets and that it could be an anti-thrombotic drug target.
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