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Blood, 1 July 2007, Vol. 110, No. 1, pp. 424-432. Prepublished online as a Blood First Edition Paper on March 22, 2007; DOI 10.1182/blood-2006-09-047480. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2006-09-047480v1 110/1/424    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Buza-Vidas, N. Articles by Sitnicka, E. Search for Related Content PubMed PubMed Citation Articles by Buza-Vidas, N. Articles by Sitnicka, E. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 14, 2006 Accepted March 9, 2007 Crucial role of FLT3 ligand in immune reconstitution following bone marrow transplantation and high dose chemotherapy Natalija Buza-Vidas, Min Cheng, Sara Duarte, Hojjatollah Nozad, Sten Eirik W Jacobsen*, and Ewa Sitnicka Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology & Cell Therapy, Lund University, Lund, Sweden * Corresponding author; email: sten.jacobsen{at}med.lu.se. Almost five decades after the first clinical transplantations, delayed immune reconstitution remains a considerable hurdle in bone marrow (BM) transplantation, and the mechanisms regulating immune reconstitution post-transplantation remain to be established. Whereas adult fms-like tyrosine kinase 3 ligand deficient (FL-/-) mice have reduced numbers of early B and T cell progenitors, they sustain close to normal levels of mature B and T cells. Herein, we demonstrate that adult BM cells fail to reconstitute B cell progenitors and conventional B cells in lethally irradiated FL-/- recipients, which also display delayed kinetics of T cell reconstitution. Similarly, FL is essential for B cell regeneration following chemotherapy-induced myeloablation. In contrast, fetal progenitors reconstitute B lymphopoiesis in FL-/- mice albeit at reduced levels. A critical role of FL in adult B lymphopoiesis is further substantiated by an age-progressive decline in peripheral conventional B cells in FL-/- mice, whereas fetally and early postnatally derived B1 and marginal zone B cells are sustained in a FL-independent manner. Thus, FL plays a crucial role in sustaining conventional B lymphopoiesis in adult mice, and as a consequence, our finding implicate a critical role of FL in promoting immune reconstitution following myeloablation and BM transplantation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. Buza-Vidas, M. Cheng, S. Duarte, H. Nozad Charoudeh, S. E. W. Jacobsen, and E. Sitnicka FLT3 receptor and ligand are dispensable for maintenance and posttransplantation expansion of mouse hematopoietic stem cells Blood, April 9, 2009; 113(15): 3453 - 3460. [Abstract] [Full Text] [PDF] L. Kenins, J. W. Gill, R. L. Boyd, G. A. Hollander, and A. Wodnar-Filipowicz Intrathymic expression of Flt3 ligand enhances thymic recovery after irradiation J. Exp. Med., March 17, 2008; 205(3): 523 - 531. [Abstract] [Full Text] [PDF]

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